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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 库存:
99
- 供应商:
联硕生物
- 规格:
500ul
保存温度:-20℃
NHWZPCIDKVYAMXOLGJBTQERSUF
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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
Inc.) or QiaFilter™ (Qiagen Inc.) plate positioned over a 96 deep-well collection block inside a vacuum manifold. Transfer 600 ul at a time, collecting the top half (with flocculent ppt.) first. Vacuum filter using approximately 300-500 mm Hg until filtration
Clonality - X Chromosome Inactivation Assay
(1:1:1, v:v:v) (Gibco) 3 M Na Acetate, pH 5.2 (Life Technologies) Isopropanol Glycogen, 10 mg/ml (GenHunter) 70% ethanol Buffer 1 (50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 50 mM NaCl) Hha I restriction enzyme (Life Technologies) REact 2 Buffer
【原创】我做染色质免疫沉淀的一些体会----续 核酸版2005年3月份明星技术: ChIP
glycogen (final concentration) 6. resuspend DNA in 20 ul TE 7. treat with 40 ug/ml RNAse for 1hr at 37 degrees C 8. run half of each sample on a 1.5% agarose gel with EtBr with an appropriate DNA sizing ladder (sheared DNA should run between 100 bp
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