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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
Chrysin 6-C-arabinoside-8-C-glucoside
- 库存:
99
- 供应商:
联硕生物
- CAS号:
185145-33-9
- 规格:
5mg
纯度:HPLC≥90%
保存温度:2-8℃
英文名称:Chrysin 6-C-arabinoside-8-C-glucoside
分子式:C26H28O13
QBGXPJWTFVZIDECUKMRYLHANSO
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文献和实验Crystallization of Kinesin Family Motor Proteins
/v) PEG 4000, 0.3% octyl-ß-D-glucoside, 2 mM ATP, 10 mM MgCl2Reservoir: 15% (w/v) PEG 4000, 60 mM NaCl equally bufferedSablin & Fletterick, 1995Sablin et al., 1996Ncd 293-700Hanging drop 18°C17 mg/ml protein in 20 mM HEPES pH 7.4, 200 mM NaCl, 10 mM
Phycoerythrin conjugation protocol
30 µl DTT (0.5M, i.e. 77 mg/ml in PBS). Incubate for 30 mins at room temperature. Separate derivatized PE and Ab from activators on G-10 columns. You should now have approximately 2 ml of each reactant. Mix derivatized
Single tube confirmation PCR protocol
(200mg/l). - Incubate at 30°C for two to three days. *This step reduces background by eliminating aborted transformants. 2. Zymolyase treatment - Transfer a small portion of a well-isolated colony (~1 mm) into 50 µl solution
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