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- AMEKO
- 中国
- AB62988-5mg
- 2025年08月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8℃
- 英文名：
Calycanthoside
- 库存：
99
- 供应商：
联硕生物
- CAS号：
483-91-0
- 规格：
5mg
纯度：HPLC≥95%
保存温度：2-8℃
英文名称：Calycanthoside
分子式：C17H20O10
FGEUHCBWQYILVPOAKNJZSRTDXM
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文献和实验Methods for use with the mTn-lacZ/LEU2-mutagenized library
is grown to a density of 107 cells/ml (O.D. 600 of 1). Use of such logarithmically-dividing cultures increases transformation efficiency. Cells are pelleted and washed once with 5 volumes of One Step buffer (0.2M LiAc, 40% PEG 4000, 100 mM beta
CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST
down in Sorvall--5 K, 5 minutes, 4℃. Drain. Resuspend cells in 5 mL HEPES/sorb to wash. Spin down (5 K, S minutes, 4℃). Drain. Resuspend in 5 mL HEPES/sorb with 0.5 mM PMSF. Add 30-40 mL 1 mg/mL oxalyticase (can also use 2 mg zymolyase here, as preferred
Yeast Chromatin Immunoprecipitation (ChIP)
ice, and store at �70 degrees C Day 5 1. Thaw extracts on ice and transfer a volume of extract (1mg total protein) to a new 1.5 ml tube for IP 2. Include necessary controls (i.e. no Ab control; untagged strain + Ab) 3. Bring all 1mg aliquots
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