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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
Pratensein 7-O-glucopyranoside
- 库存:
99
- 供应商:
联硕生物
- CAS号:
36191-03-4
- 规格:
5mg
纯度:HPLC≥98%
保存温度:2-8℃
英文名称:Pratensein 7-O-glucopyranoside
分子式:C22H22O11
ZVHLDBKXQNSUOGRMFIPJWYACET
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文献和实验CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST
down in Sorvall--5 K, 5 minutes, 4℃. Drain. Resuspend cells in 5 mL HEPES/sorb to wash. Spin down (5 K, S minutes, 4℃). Drain. Resuspend in 5 mL HEPES/sorb with 0.5 mM PMSF. Add 30-40 mL 1 mg/mL oxalyticase (can also use 2 mg zymolyase here, as preferred
Naito, S. and Shimamoto, K. (2000) Post-transcriptional gene silencing in cultured rice cells.Plant Cell Physiol. 4 1 , 321 -3 26 . 3. F ire, A ., X u , S. Q ., M ontgomery, M. K ., K o stas, S. A ., D river, S. E. and
Before--Subculture overnight into 110 mL (large flask for aeration). Dilute so ~2x107 cells/mL at convenient time on Day 1, or if a cell cycle arrest will be performed, subculture into 220 mL and have cells at ~O.D. 0.5 at the time of addition of alpha factor, HU
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