- ￥34.20
- AMEKO
- 中国
- AB66469-500mg
- 2025年08月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8℃
- 英文名：
N-CARBAMYL-L-GLUTAMIC ACID
- 库存：
99
- 供应商：
鹿森生物
- CAS号：
1188-38-1
- 规格：
500mg
纯度：98%
保存温度：2-8℃
英文名称：N-CARBAMYL-L-GLUTAMIC ACID
分子式：C6H10N2O5
CXOJESAPFWRMUDZTBKVIQNGHYL
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文献和实验ELISA (Enzyme-Linked ImmunoadSorbent Assay)
with 1x PBS containing 0.01% tween. 9) Add peroxidase conjugate (50ul/well) diluted 1:1000 with 1% BSA (diluted in washing solution as in 8)) and incubate for 1hr @ 37°C. N.B.: Use PRAM (Peroxidase-conjugated Rabbit Anti-Mouse) for Ab raised in mice
(交流)ELISA (Enzyme-Linked ImmunoadSorbent Assay)
(50ul/well) diluted 1:1000 with 1% BSA (diluted in washing solution as in 8)) and incubate for 1hr @ 37°C.N.B.: Use PRAM (Peroxidase-conjugated Rabbit Anti-Mouse) for Ab raised in mice and PSAR (Peroxidase-conjugated Swine Anti Rabbit) for Ab raised
mlAdd 40 g ammonium sulfate for every 100 ml Sup. (slowly):800gStir O/N in cold room.Pour into plastic bottles. Spin at 4 ℃, 7,500 rpm for 20 min in JA-10 rotor.Prepare 4L 100 mM Tris/pH7.8 (400 ml of 1 M stock/4 L) or Binding buffer.Discard Sup. Resuspend
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