- ￥140.22
- AMEKO
- 中国
- AB65915-100mg
- 2025年08月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 英文名：
Gly-DL-Asp
- 库存：
99
- 供应商：
鹿森生物
- CAS号：
79731-35-4
- 规格：
100mg
纯度：≧95%
保存温度：-20℃
英文名称：Gly-DL-Asp
分子式：C6H10N2O5
VZMBGNDOAWYFCPQKISELTHXRJU
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文献和实验Factor Xa Cleavage of MBP-Fusion protein
for each system. Samples must be removed at various time points and analyzed by PAGE-SDS to estimate the yield, purity and extent of factor Xa digestion. Factor Xa cleaves after the Arg residue in the cleavage site Ile-(Glu or Asp)-Gly-Arg. Ile and Thr are prefered
Preparing a Selenomethionyl Protein
50x stock Use all amino acids EXCEPT Gly, Ala, Pro, Asn, Cys, and Met at a concentration of 2mg/ml To help in dissolving the amino acids, autoclave for 10 minutes. 20% glucose (sterile filtered or autoclaved) 1M MgSO4 (sterile
Preparing a Selenomethionyl Protein
and grow until an OD600 =0.5-0.6. (~2.0 - 2.5 hours) Add 100mg threonine, lysine hydrochloride, phenylalanine to the culture. Add 50mg leucine, isoleucine, valine to the culture (all as solid powders). Add 120mg DL-Se-Met or 60mg L-Se-Met
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