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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 库存:
99
- 供应商:
鹿森生物
- 规格:
500ml
纯度:5×,pH6.8,无菌
保存温度:RT
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文献和实验Gentle hypochlorite treatment 1. Wash off plates of gravid hermaphrodites with 10-15 mls of M9 and put in 15 ml conical tube. Collect minimum of 4 or 5 plates in a tube. 2. Centrifuge @500rpm for about 3 minutes.
Expression Protocol in M9 Minimal Media via T7 Promoter
For 1L 5xM9 salts: 64g Na2 HPO4 -7H2 O 15g KH2 PO4 2.5g NaCl H2 O to final 1L volume and autoclave To prepare 500mL M9 minimal media; 100mL of 5xM9 salts 1 mL 1M MgSO4 50 uL 1M CaCl2 5 mL 100x Basal
Expression Protocol in M9 Minimal Media via T7 Promoter
NaCl H2O to final 1L volume and autoclave To prepare 500mL M9 minimal media; 100mL of 5xM9 salts 1 mL 1M MgSO4 50 uL 1M CaCl2 5 mL 100x Basal Medium Eagle Vitamin Solution (Gibco) 2.5 mL filter sterilized NH
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