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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
Hetisine hydrochloride
- 库存:
99
- 供应商:
鹿森生物
- CAS号:
149926-20-5
- 规格:
5mg
纯度:95%
保存温度:2-8℃
英文名称:Hetisine hydrochloride
分子式:C20H27NO3·ClH
MUCXSFEKHLOIGYVZDANRBWPTJQ
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文献和实验Conjugation of Antibody to HRP
Antibody Concentrate the antibody to 5-7 mg/ml Dissolve 10 mg of SATA in 1 ml DMF (SATA:DMF) Add 3.0 ml SATA:DMF per mg of antibody Gently stir solution for 2 hours at room temperature. Dissolve 0.5 g hydroxylamine hydrochloride in 10 ml PBS
Conjugation of Antibody to HRP
the first peak. Preparation of Ab:HRP Conjugate Immediately mix the HRP with the thiolated antibody at 1 mg HRP per mg antibody. Gently stir for 16-20 hours at 2-8o C
ELISA (Enzyme-Linked ImmunoadSorbent Assay)
and PSAR (Peroxidase-conjugated Swine Anti Rabbit) for Ab raised in rabbits. 10) Wash 4x with 1x PBS containing 0.01% tween. 11) Add substrate (100ul/well). 12) Incubate for 1-5 mins in the dark at RT. Check reaction for colour change. Wells
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