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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 英文名:
Esculentoside S
- 库存:
99
- 供应商:
鹿森生物
- CAS号:
156031-83-3
- 规格:
5mg
纯度:HPLC≥98%
保存温度:-20℃
英文名称:Esculentoside S
分子式:C42H66O16
LHWIRMDAZXOCGQSJFTUEBNKVYP
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文献和实验Phycoerythrin conjugation protocol
Phycoerythrin conjugation protocol David's method modified from references (2) and (3). I used this method to conjugate a mouse IgG2a monoclonal antibody (IB4). It works well. Reagents Ab Antibody
DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
) through the oil to sample. (It's 0.3 ul per 15ul original volume, so adjust as necessary). Incubate overnight at 55 C. Day 3: (Repeat:) Add 0.3 ul fresh conc Proteinase K (20 mg/ml stock) through the oil to sample. (It's 0.3 ul per 15 ul original
ELISA (Enzyme-Linked ImmunoadSorbent Assa
-conjugated R abbit A nti-M ouse) for Ab raised in mice and PSAR (P eroxidase-conjugated S wine A nti R abbit) for Ab raised in rabbits. 10) Wash 4x with 1x PBS containing 0.01% tween. 11) Add substrate (100ul/well). 12) Incubate for 1-5 mins
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