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- 保存条件:
2-8°C
- 保质期:
6个月
- 库存:
100
- 供应商:
百欧泰
- 规格:
10mg/ml
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文献和实验Preparation of Fluorescent DNA Probe from mRNA: Yeast
Millipore filter, 0.45 um 1 Millipore #UFC3OHVNB . *50X dNTPs stock Final conc. dATP (100 mM) 25 uL 25 mM dCTP
Preparation of Fluorescent DNA Probe from mRNA: Yeast
50X dNTPs 0.6 1.25 500 uM; 200 uM dTTP Cy3- or Cy5 dUTP 3.0 --- 100 uM Superscript II (200 U/uL) 2.0
Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye terminator reactions
One of the major problems in DNA cycle sequencing is that when fluorescent primers (1) are used the reaction conditions are such that the nested fragment set distribution is highly dependent upon the template concentration
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