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- 文献和实验
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- 保存条件:
2-8℃
- 保质期:
6个月
- 库存:
100
- 供应商:
百欧泰
- 规格:
10 ml
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
. x. Place 50 µL of the biotin-avidin-HRP mix on the preparation and cover with a coverslip. Incubate the slides for 40 min at room temperature in a humid chamber. xi. Wash slides in wash solution 1 for 15 min
, Biotin Blot Total Protein Stain, or an anionic dye such as Amido Black. Zeta-Probe membrane can be stained with the Biotin-Blot Total Protein Stain.SDS may be added to Buffer 1 to increase protein elution from the gel:48mM Tris, 39 mM glycine, (20
, prestained molecular weight standards can be used, or a portion of the membrane can be stained for total protein with colloidal gold, Biotin Blot Total Protein Stain, or an anionic dye such as Amido Black. Zeta-Probe membrane can be stained with the Biotin
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