K112693P Anti-DYRK3 Polyclonal

【抗体靶点简介】

Dual-specificity protein kinase that promotes disassembly of several types of membraneless organelles during mitosis, such as stress granules, nuclear speckles and pericentriolar material (PubMed:29973724). Dual-specificity tyrosine-regulated kinases (DYRKs) autophosphorylate a critical tyrosine residue in their activation loop and phosphorylate their substrate on serine and threonine residues (PubMed:9748265, PubMed:29634919). Acts as a central dissolvase of membraneless organelles during the G2-to-M transition, after the nuclear-envelope breakdown: acts by mediating phosphorylation of multiple serine and threonine residues in unstructured domains of proteins, such as SRRM1 and PCM1 (PubMed:29973724). Does not mediate disassembly of all membraneless organelles: disassembly of P-body and nucleolus is not regulated by DYRK3 (PubMed:29973724). Dissolution of membraneless organelles at the onset of mitosis is also required to release mitotic regulators, such as ZNF207, from liquid-unmixed organelles where they are sequestered and keep them dissolved during mitosis (PubMed:29973724). Regulates mTORC1 by mediating the dissolution of stress granules: during stressful conditions, DYRK3 partitions from the cytosol to the stress granule, together with mTORC1 components, which prevents mTORC1 signaling (PubMed:23415227). When stress signals are gone, the kinase activity of DYRK3 is required for the dissolution of stress granule and mTORC1 relocation to the cytosol: acts by mediating the phosphorylation of the mTORC1 inhibitor AKT1S1, allowing full reactivation of mTORC1 signaling (PubMed:23415227). Also acts as a negative regulator of EPO-dependent erythropoiesis: may place an upper limit on red cell production during stress erythropoiesis (PubMed:10779429). Inhibits cell death due to cytokine withdrawal in hematopoietic progenitor cells (PubMed:10779429). Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1: this in turn inhibits p53/TP53 activity and apoptosis (PubMed:20167603).

【实验操作视频参考】

Western blot实验参考操作流程

Immunohistochemistry实验参考操作流程

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索莱宝现有30000余种经过严格实验验证的抗体产品，全面覆盖神经科学、细胞凋亡、表观遗传学等热门研究方向，适配 WB、IHC、ELISA、IP、co-IP 等主流实验技术场景。包括内参标签抗体，以及多种标记类型的一抗、二抗，充分满足客户多样化实验选型需求。索莱宝拥有成熟专业的抗体制备与质量检测平台，从免疫制备、纯化提纯到实验验证全流程严格筛选评估，全方位保障每一支抗体的性能与品质。

此外，索莱宝已完善一站式技术服务平台，可提供 WB、IHC、IF、IP、流式检测、细胞实验等常规检测，以及多克隆/单克隆抗体定制等个性化定制服务，为科研用户提供全方位解决方案。