Anti-DYRK3 Polyclonal Antibody

产品简介

中文名称	双特异性酪氨酸-(Y)-磷酸化调节激酶3抗体
靶标	DYRK3
别名	Dual specificity tyrosine (Y) phosphorylation regulated kinase 3; Dual specificity tyrosine phosphorylation-regulated kinase 3; Dual specificity tyrosine-phosphorylation-regulated kinase 3; Dyrk3; DYRK3_HUMAN; DYRK5; hYAK3 2; Protein kinase Dyrk3; RED; REDK; Regulatory erythroid kinase;
背景资料	Dual-specificity protein kinase that promotes disassembly of several types of membraneless organelles during mitosis, such as stress granules, nuclear speckles and pericentriolar material (PubMed:29973724). Dual-specificity tyrosine-regulated kinases (DYRKs) autophosphorylate a critical tyrosine residue in their activation loop and phosphorylate their substrate on serine and threonine residues (PubMed:9748265, PubMed:29634919). Acts as a central dissolvase of membraneless organelles during the G2-to-M transition, after the nuclear-envelope breakdown: acts by mediating phosphorylation of multiple serine and threonine residues in unstructured domains of proteins, such as SRRM1 and PCM1 (PubMed:29973724). Does not mediate disassembly of all membraneless organelles: disassembly of P-body and nucleolus is not regulated by DYRK3 (PubMed:29973724). Dissolution of membraneless organelles at the onset of mitosis is also required to release mitotic regulators, such as ZNF207, from liquid-unmixed organelles where they are sequestered and keep them dissolved during mitosis (PubMed:29973724). Regulates mTORC1 by mediating the dissolution of stress granules: during stressful conditions, DYRK3 partitions from the cytosol to the stress granule, together with mTORC1 components, which prevents mTORC1 signaling (PubMed:23415227). When stress signals are gone, the kinase activity of DYRK3 is required for the dissolution of stress granule and mTORC1 relocation to the cytosol: acts by mediating the phosphorylation of the mTORC1 inhibitor AKT1S1, allowing full reactivation of mTORC1 signaling (PubMed:23415227). Also acts as a negative regulator of EPO-dependent erythropoiesis: may place an upper limit on red cell production during stress erythropoiesis (PubMed:10779429). Inhibits cell death due to cytokine withdrawal in hematopoietic progenitor cells (PubMed:10779429). Promotes cell survival upon genotoxic stress through phosphorylation of SIRT1: this in turn inhibits p53/TP53 activity and apoptosis (PubMed:20167603).
Swiss Prot	O43781
亚细胞定位	Cytoplasm Nucleus
宿主	Rabbit
克隆类型	Polyclonal Antibody
亚型	IgG
预测交叉反应	Monkey
蛋白分子量	64/66kDa
实际分子量	66kDa
稀释比例	WB 1:2000-5000. IHC 1:50-200.
免疫原	Recombinant protein of human DYRK3
Public Immunogen Range	200-523aa
纯化方法	Affinity purification
储存液	Buffer: PBS with 0.03% Proclin300, 50% glycerol, pH7.3.
保存	Store at -20℃. Avoid freeze / thaw cycles.
交叉反应	Human;Mouse;Rat
应用	WB;IHC
规格	50ul 100ul
单位	支

备注：
以上数据均来自公开文献, Solarbio暂未进行独立验证, 仅供参考。
These protocols are for reference only. Solarbio does not independently validate these methods.
温馨提示：
使用前请离心。如有问题请及时联系我们。
Quick spin before use. If you have any questions, please contact us.
实验图

Western blot analysis with DYRK3 antibody diluted at 1:3000;Lane: Raji.

Immunohistochemistry of paraffin-embedded human testis cancer with DYRK3 antibody diluted at 1:100.