AXMIR-124a RNA oligo anti-miRN

RNA芯片

人类染色体上基因序列测定的结果提示人类基因组中基因的数量可能要远远小于以前的推测，也提示基因功能可能存在多样性。研究一个基因在不同的组织中的表达情况有助于了解基因功能的多样性。想研究某个基因在人体内各种组织中的表达情况，RNA芯片将是最合适的选择。 这种芯片是真正的芯片，玻璃片基，上面点有从总共124种胎儿、成人组织（含20种肿瘤细胞株）中提取的高质量的mRNA。 只要将待检测的基因制备成探针，与RNA芯片进行一次杂交，即可得到这个基因在124种

Hybridisation to RNA in Tissue Sections

polymerase to generate both the sense and anti-sense strands (and eliminate variability between the T7 and T3 RNA polymerases - one way to try to match sense against anti-sense). The template must be linearised when undertaking in vitro transcription

小RNA的Northern BLOT

if you own one!), add Kodak MS film (greater sensitivity with intensifier screen) and expose blot at -80C.  I usually do a 1-3 hour exposure to get an idea of how long I will need to expose. For really highly expressed miRNA s (i.e. miR-124, may only need 1 hour