pCDH-CuO-MCS empty cloning len

Maxiprep preparation of Plasmid DNA

实验概要 The PureLink™ HiPure Plasmid DNA Maxiprep Kit allows purification of 500–850 μg of high-quality plasmid DNA from 100–200 mL overnight E. coli cultures in ~2 hours when cloning high copy number plasmids.

PCR的下游应用

Purification with Sepahcryl S-300 or S-500 - in filter bottom plates  96-well silent screen plate, Purification with Guanidine-HCl in MES buffer - in glass filter plates Purification with Guanidine-HCl in KAc buffer    Extraction of PCR Oil Using

CORE SAMPLE PCR

while maintaining yield, and attempting to purify the band by cloning all the reaction products and then probing the library for the correct DNA can be extraordinarily tedious. I have applied a simple "core sampling" procedure - involving "coring" an agarose