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- NM_001166163.1(1点突变);Neurabin-I; NRB1; NRBI
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- 2025年08月11日
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- 详细信息
- 文献和实验
- 技术资料
- 提供商：
上海博尔森生物科技有限公司
- 服务名称：
pCR-XL-TOPO-PPP1R9A(human)(1点突变)
- 规格：
干粉质粒
pCR-XL-TOPO-PPP1R9A(human)(1点突变)
[ 产品信息 ]
[ 质粒简介 ]
pCR-XL-TOPO-PPP1R9A(human)(1点突变)
[ 参考序列 ]
GCGTTAGAATACTCAAGCTATGCATCAAGCTTGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTATTTGTAGAGGGCAGGAAGAGGTGGGGACTTCTCTTGGAGCATCTCCATCATCTGTAAGAGGGTATGTGTTACTGCTCACCAGCACCCTCGGCTGTAGTTGACGTCATCTTTTCTGTCTTTTTGGACTTCCTCTGCATTTGCTCTTGCTCCTTTCTCCTTAGCTTTTCTCTTTGTTTTTCCATTTTCTCTTGGGCCTTCCGAGCCTTCTCTAGAGACATCTTCATTTCCTTGAGTTTCTTTTTGACCACTGCTCGGTCCTGGGATGCTGTCATTCCAAGAGCCTTAAGTTTATTTCCATCCAACTGCAGGAGCTGTTCTCCAGTGATGTTTTGGGCACTGAATTCAGATACATACTGCTCCAGATTTAGGCTCATTAACCATTGAGAAACCTGCTGCACACTCCATTCCTGAACGGCCCGATTCTGACACTGACTGTGTTTGGGAGACTGTCCATCATCAAGGATCTCATCTGATGACAGTGCTAAGGACTGAGAGAGCCCTGGTGTTTTAGGTTCTGCTCCTAAGCCGCTGAGGTCTGCTGAACTGGTACTGCTGGGACTGAAGTCATCATTGAAGGTAAAATTCTTTCTCTCTAGACTGTCCTCCCCATCTGTGGAACTAACACTATCATACAGTCTTGTCCTAGAGGGTCTCTGGCGTCTGTTCTTCACAGAGAGCTGGGCTCGAGTTTTCAGTGCTTTTGAATCCAGGCGCTCTGTCTCTGGGACTGCTTCATTCAAGTCTTGACTTAGGCCATCTTGGCAAGATGTCTGCTTGCCATCCAAGTTCTCCATGGTATCCCCTTTAGAGACTTCACCAAGAGATGTTCTTCTCTCAAAGATGTTGTTATTATTGTTAACTTGAGTCCCATTTTGCTTCAGTAGCCTGAATACCTCAGCTTCCAAATCTCTAATCCGCACTTGGAGGCCTTGCACTTCCACAAGATGAGCTTTTTCCAAATCTTCTATTTTCTTTCTTTCTGCTTCCTGTCTTTTGATGAAATCAAGCTCTTTTTGTTGAAAATCCTTGATCAACTTCTTTGCCTTGTTGTATTTCTTTTCCAAGGCCTGATACTGGCTTTGAGTCTCTTTGAGATGCTCATTCACTGTGTGGCATAATGTTTGGGCCTCAATCCAGTAGCTTTCCAACTTCAACATTCTTTCCTTATTCTCTTCTATGTTTTGTTGGAGTTGGGTTTTTTCTAGTTCCCACCTCACTTTCTCATTTTCTGCTGCCTGCAGCTTGGTCTTCAATTTTTGAATCTCTGCTTCTGTAACTGCATGTTTGATTTGCAACTCTTTGAACTTGTGACTGAGCTTGCTTGTGTCCAGTTCTGATGGGGAAAACATGTCCTCATTCTCAGGCAGCTCAAAGACTTCAATGGCCATGTCGCTGCCAGGAAGGACAGGTCCTACCTCATCTTCTTCTTCATCTGTGGCATATTCTCCTGTCTCGTCATCGTCGGCATCATACTGGGCATAGTGCTGTTCCAGCAGCTCTCTCTGGCGCCTCTCCTGTTCCAGTGTCTGGCTTATCAACTGGGCAACCTCGCTCACTTGTCCTGGTTTTTCCCGCCCAATAACAAATCTGACGTTGCCCTTGGTGTTTCTGAGAACTGTTGCTGCAAAATTCTGTGTCACACCCACCAAGCTGATTCCATCCACTTCCACAATCTGGTCATTGACTTGTATTCTGCCATCCCGTTGAGCAGCACCACCTTCTGTTACTGTCTTGACGAATATTCCCAGCTTTTCAAGTCCAGCATCTGCTCCAACACCCATTCCAATAATACTTATACCAAGACCATCCTCATCTTTCTCTAGCTCCACTGGGAAAAGTTCCAGCTTTTCTACACGTTTTTCAAGTTCATACTCAGCTGAAGCAGCCACAGGGTCAACTTCGTCATTTCTCCTGTCATAGTCTTCATTGGAGTATGTGTTGAAAACCTTAATAGGAGCACTACTAAACTTAATTTTCCTATTTGCTGGGATTTCTTCTTCTTCTGGCAATCCAACAATTTCCGAGTACTCCATATCAGGCTGATAGTAACTGTTCTCATCACTATCTTCCTCCTCATCCTGCTCAGTGCCTGTCTCTCCCCAGTCTGAATTATACCTGGATCTCACCCTATACACATTATAGTCACTGTGCATGTACACATGGGAACCATCAAAATTATTTGAATCTTCAGGTACTTCTTTTCCACAACTGGATGCAGAAGCATCAGGAGAGGTGAAATCACCACCTGCAAGTTCTTTCCTCTGTTGCTTTGCTGCCTCCCTTCCAACCAAATTAGCTTCAGCATCTTCTAACAGTTGGCTTTGTGGTGAAGGGATTTCGGACTTTGGCATTGCCTTACTTTCAGCACAAGGTTCTTCAGGACCATCTTTGTCAATGCTGTCGGGAGTCTGTTGATTAGATGTGGAGTCCTCGGGTTCCTTGCTCTGCTGGATCTCTTCACCAGGTATCGAAGCTAGAGAGGTACTTTTAGAAGCCACTTCTGGTACTGGAGTTGCATTACTCTTGTGAGCATCCTCTGTATCAACACCTCGCTTGTTTGAAGGAGGCCAGGAATTAGAATCCTTCAGGTGACCAAATGTGTCAAGATTTGTAACAGTAACAGATGGTAAATTCAAGGGATAATGCCCAGTCACTGAGTATTCATTGTTTTCAGCCTTCTCAGAAATGATGGCACTGGGAGAATCAGTGTTCTCAAATACTGCACTCAGTTGACTCACAGTTGGGGAGACAGCCTCAGTTCGGGAGCTAAGGCTGTCCAAGGAATCAGTACTGCCTCTGTTGGACTTGGAACCTCCCCATTCATCCTGAGGTTCACTCCCTCCAGCTTTCTCTTTCTTTGGGGAATAGCGGTTGTTCTGTCCTGATTCATGCACACTTCTCTCAAACATCTTTCGAGTCTCAGTGAACTTGGAATATGAAGGGCCATCGTACATAGTGTCAAATCTACTAATTCGTTCAGAAACAGAAGACTCCAACTTAACAACTGAGCCATCTGTTTTTTCCAGAAATTCTTTGGGCTTCATTCTTCTCTGAGGAGATGAATGTCCACCTTTCCCCCTTGTTTTGGCAATGACTGCAGCATTCTCGTTGGGTTCCATACCCATCTGCATAAATAGGTTTTTAATTCTGTTGACATTGGAGCCATATTTCCTCCCCCTGCTCTGCTGGGAGCCCTCACCTTCTTTTGTTTTTTGTTCCCCATCTGACTTGGGTTTGTCAAAGGTACTTTTCAGTGCCTGAAACTCAGTTCGATATGCATTCCTGTGAGGAGAGGCACTTCTGAGAGTGGTTCGTTCACCTGAAGACTCAGTTTTCAACATTTTCACTTCAGGGGTGAAAAGCCAATGTTCAAAGGGCGAATTCTGCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCAATCGCC
注意事项：该产品仅供科研实验使用，未经允许不得用于其它用途！以实际收货产品说明书为准，网站说明书仅供参考。
[ 产品信息 ]
| 名称 | pCR-XL-TOPO-PPP1R9A(human)(1点突变) | 别称 | NM_001166163.1(1点突变);Neurabin-I; NRB1; NRBI |
|---|---|---|---|
| 基因长 | 3273bp | 质粒宿主 | 大肠杆菌 |
| 质粒用途 | 基因模板 | 片段类型 | cDNA |
| 片段物种 | 人 | 原核抗性 | Kan |
| 货号: | BES226450PVE | 荧光标记 | |
| 启动子 | 复制子 | pUC | |
| 感受态 | DH5a | 温度 | 37度 |
| 背景载体 | pCR-XL-TOPO | 正向引物 | M13F：TGTAAAACGACGGCCAGT |
| 反向引物 | M13R：CAGGAAACAGCTATGACC | 拷贝数 |
pCR-XL-TOPO-PPP1R9A(human)(1点突变)
[ 参考序列 ]
GCGTTAGAATACTCAAGCTATGCATCAAGCTTGGTACCGAGCTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTATTTGTAGAGGGCAGGAAGAGGTGGGGACTTCTCTTGGAGCATCTCCATCATCTGTAAGAGGGTATGTGTTACTGCTCACCAGCACCCTCGGCTGTAGTTGACGTCATCTTTTCTGTCTTTTTGGACTTCCTCTGCATTTGCTCTTGCTCCTTTCTCCTTAGCTTTTCTCTTTGTTTTTCCATTTTCTCTTGGGCCTTCCGAGCCTTCTCTAGAGACATCTTCATTTCCTTGAGTTTCTTTTTGACCACTGCTCGGTCCTGGGATGCTGTCATTCCAAGAGCCTTAAGTTTATTTCCATCCAACTGCAGGAGCTGTTCTCCAGTGATGTTTTGGGCACTGAATTCAGATACATACTGCTCCAGATTTAGGCTCATTAACCATTGAGAAACCTGCTGCACACTCCATTCCTGAACGGCCCGATTCTGACACTGACTGTGTTTGGGAGACTGTCCATCATCAAGGATCTCATCTGATGACAGTGCTAAGGACTGAGAGAGCCCTGGTGTTTTAGGTTCTGCTCCTAAGCCGCTGAGGTCTGCTGAACTGGTACTGCTGGGACTGAAGTCATCATTGAAGGTAAAATTCTTTCTCTCTAGACTGTCCTCCCCATCTGTGGAACTAACACTATCATACAGTCTTGTCCTAGAGGGTCTCTGGCGTCTGTTCTTCACAGAGAGCTGGGCTCGAGTTTTCAGTGCTTTTGAATCCAGGCGCTCTGTCTCTGGGACTGCTTCATTCAAGTCTTGACTTAGGCCATCTTGGCAAGATGTCTGCTTGCCATCCAAGTTCTCCATGGTATCCCCTTTAGAGACTTCACCAAGAGATGTTCTTCTCTCAAAGATGTTGTTATTATTGTTAACTTGAGTCCCATTTTGCTTCAGTAGCCTGAATACCTCAGCTTCCAAATCTCTAATCCGCACTTGGAGGCCTTGCACTTCCACAAGATGAGCTTTTTCCAAATCTTCTATTTTCTTTCTTTCTGCTTCCTGTCTTTTGATGAAATCAAGCTCTTTTTGTTGAAAATCCTTGATCAACTTCTTTGCCTTGTTGTATTTCTTTTCCAAGGCCTGATACTGGCTTTGAGTCTCTTTGAGATGCTCATTCACTGTGTGGCATAATGTTTGGGCCTCAATCCAGTAGCTTTCCAACTTCAACATTCTTTCCTTATTCTCTTCTATGTTTTGTTGGAGTTGGGTTTTTTCTAGTTCCCACCTCACTTTCTCATTTTCTGCTGCCTGCAGCTTGGTCTTCAATTTTTGAATCTCTGCTTCTGTAACTGCATGTTTGATTTGCAACTCTTTGAACTTGTGACTGAGCTTGCTTGTGTCCAGTTCTGATGGGGAAAACATGTCCTCATTCTCAGGCAGCTCAAAGACTTCAATGGCCATGTCGCTGCCAGGAAGGACAGGTCCTACCTCATCTTCTTCTTCATCTGTGGCATATTCTCCTGTCTCGTCATCGTCGGCATCATACTGGGCATAGTGCTGTTCCAGCAGCTCTCTCTGGCGCCTCTCCTGTTCCAGTGTCTGGCTTATCAACTGGGCAACCTCGCTCACTTGTCCTGGTTTTTCCCGCCCAATAACAAATCTGACGTTGCCCTTGGTGTTTCTGAGAACTGTTGCTGCAAAATTCTGTGTCACACCCACCAAGCTGATTCCATCCACTTCCACAATCTGGTCATTGACTTGTATTCTGCCATCCCGTTGAGCAGCACCACCTTCTGTTACTGTCTTGACGAATATTCCCAGCTTTTCAAGTCCAGCATCTGCTCCAACACCCATTCCAATAATACTTATACCAAGACCATCCTCATCTTTCTCTAGCTCCACTGGGAAAAGTTCCAGCTTTTCTACACGTTTTTCAAGTTCATACTCAGCTGAAGCAGCCACAGGGTCAACTTCGTCATTTCTCCTGTCATAGTCTTCATTGGAGTATGTGTTGAAAACCTTAATAGGAGCACTACTAAACTTAATTTTCCTATTTGCTGGGATTTCTTCTTCTTCTGGCAATCCAACAATTTCCGAGTACTCCATATCAGGCTGATAGTAACTGTTCTCATCACTATCTTCCTCCTCATCCTGCTCAGTGCCTGTCTCTCCCCAGTCTGAATTATACCTGGATCTCACCCTATACACATTATAGTCACTGTGCATGTACACATGGGAACCATCAAAATTATTTGAATCTTCAGGTACTTCTTTTCCACAACTGGATGCAGAAGCATCAGGAGAGGTGAAATCACCACCTGCAAGTTCTTTCCTCTGTTGCTTTGCTGCCTCCCTTCCAACCAAATTAGCTTCAGCATCTTCTAACAGTTGGCTTTGTGGTGAAGGGATTTCGGACTTTGGCATTGCCTTACTTTCAGCACAAGGTTCTTCAGGACCATCTTTGTCAATGCTGTCGGGAGTCTGTTGATTAGATGTGGAGTCCTCGGGTTCCTTGCTCTGCTGGATCTCTTCACCAGGTATCGAAGCTAGAGAGGTACTTTTAGAAGCCACTTCTGGTACTGGAGTTGCATTACTCTTGTGAGCATCCTCTGTATCAACACCTCGCTTGTTTGAAGGAGGCCAGGAATTAGAATCCTTCAGGTGACCAAATGTGTCAAGATTTGTAACAGTAACAGATGGTAAATTCAAGGGATAATGCCCAGTCACTGAGTATTCATTGTTTTCAGCCTTCTCAGAAATGATGGCACTGGGAGAATCAGTGTTCTCAAATACTGCACTCAGTTGACTCACAGTTGGGGAGACAGCCTCAGTTCGGGAGCTAAGGCTGTCCAAGGAATCAGTACTGCCTCTGTTGGACTTGGAACCTCCCCATTCATCCTGAGGTTCACTCCCTCCAGCTTTCTCTTTCTTTGGGGAATAGCGGTTGTTCTGTCCTGATTCATGCACACTTCTCTCAAACATCTTTCGAGTCTCAGTGAACTTGGAATATGAAGGGCCATCGTACATAGTGTCAAATCTACTAATTCGTTCAGAAACAGAAGACTCCAACTTAACAACTGAGCCATCTGTTTTTTCCAGAAATTCTTTGGGCTTCATTCTTCTCTGAGGAGATGAATGTCCACCTTTCCCCCTTGTTTTGGCAATGACTGCAGCATTCTCGTTGGGTTCCATACCCATCTGCATAAATAGGTTTTTAATTCTGTTGACATTGGAGCCATATTTCCTCCCCCTGCTCTGCTGGGAGCCCTCACCTTCTTTTGTTTTTTGTTCCCCATCTGACTTGGGTTTGTCAAAGGTACTTTTCAGTGCCTGAAACTCAGTTCGATATGCATTCCTGTGAGGAGAGGCACTTCTGAGAGTGGTTCGTTCACCTGAAGACTCAGTTTTCAACATTTTCACTTCAGGGGTGAAAAGCCAATGTTCAAAGGGCGAATTCTGCAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCAATCGCC
注意事项：该产品仅供科研实验使用，未经允许不得用于其它用途！以实际收货产品说明书为准，网站说明书仅供参考。
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文献和实验相关实验
XL PCR Amplification of Long Targets from Genomic DNA
Long polymerase chain reaction (PCR)>(1 5 ), specifically, XL PCR (Extra-Long Polymerase Chain Reaction), has enabled amplification of expanded trinucleotide repeats of the neuromuscular disease myotonic dystrophy (6 ) a 9-kb HIV
的高保真聚合酶之一，堪称高保真聚合酶的“黄金标准”，是Stratagene的看家之宝，能够有效避免延伸过程中不需要的错配。试剂盒采用的是低次数的循环延伸而非PCR，有助于减少无意错配。只需要一次酶切和转化，实验可以在一天完成。这个试剂盒适用于质粒大小不超过8Kb的质粒。后来推出的QuikChange XL site-directed mutagenesis kit则是针对大于8Kb的质粒的定点突变的，通过优化试剂特别是其感受态细胞（XL10-Gold），使得较大的质粒的定点突变也一样简单
大小不超过8Kb的质粒。后来推出的QuikChange XL site-directed mutagenesis kit则是针对大于8Kb的质粒的定点突变的，通过优化试剂特别是其感受态细胞（XL10-Gold ），使得较大的质粒的定点突变也一样简单。QuikChange由于操作简单快速，效率高（>80%）而受到普遍欢迎，光是今年的前9个月就有超过400篇发表的论文中用到这个产品。 定点突变的比较有特色的产品还有Clontech公司的Transformer Site-Directed
pCR-XL-TOPO-PPP1R9A(human)(1点突变)
￥680
