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- 文献和实验
- 技术资料
- 英文名:
3,5-Difluoro-4-methoxybenzeneboronic acid pinacol ester
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
890839-37-9
- 规格:
10mg///100mg///200mg
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文献和实验Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
fresh 10 mg/ml stock in DMSO.Need 2.5 mg per 50 mg of tubulin. 8.Potassium glutamate,1 M.Prepare 1 M glutamic acid and titrate with KOH to pH 6.9. 9.Injection Buffer: 50 mM potassium glutamate,0.5 mM MgCl2,pH 6.5-6.7.Prepare 10 ml.Keep ~5 ml at 37℃
Labeling Tubulin and Quantifying Labeling Stoichiometry
(warm = 37¡C) TLA100.4 or TLA100.3 and TLA100.2 rotors Small Dounce (2 ml) Note : 1M HEPES, pH to 8.6 with NaOH and store at -20¡C 2M K-Glutamate - dissolve glutamic acid to 2M, carefully pH with KOH such that 50 mM has a pH
Preparation of Carboxypeptidase Y and Its Properties
】 Carboxypeptidase Y is a kind of proteases in bread yeast. It has the ability to remove most amino acid residue, including that of proline, from the C-termini of proteins and peptides. These unique properties render a useful tool in C-termini
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