1,1′-双(二环己基膦)铁二氯化钯(II) 1,1′-B

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for synthesizing cyclic bis(3′‐5′)diguanylic acid (c‐di‐GMP). Bull. Chem. Soc. Jpn. 77:2089‐2093.    Hyodo, M., Sato, Y., and Hayakawa, Y. 2006. Synthesis of cyclic bis(3′‐5′)diguanylic

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View Image   Figure 1.30.5 Synthesis of nucleoside 5′‐(α‐ P ‐thio)triphosphates utilizing sulfurizing reagent II as the oxidizing agent

miRNA的靶基因的寻找与鉴定

信息学的方式进行，主要是根据以下几个方面进行预测：①miRNA与靶基因结合位点的互补性；②miRNA靶位点在不同物种之间的保守性；③miRNA-mRNA结合的热稳定性；④miRNA靶位点处不应有复杂二级结构；⑤miRNA5′端与靶基因的结合能力强于3′端。除以上几个基本原则外，不同的预测方法还会根据各自总结的规律对算法进行不同的限制与优化。目前比较常用的靶基因预测软件有：miRanda、TargetScan、PicTar、miRWalk、miRanda、miRDB等，其中在miRWalk中可以查找到已经