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VSV-G-tag Antibody, pAb, Rabbi

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  • ¥990
  • GenScript
  • A00199-40
  • 2026年05月28日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商

      广州市左克生物

    • 库存

      充足

    • 保存条件

      4ºC

    • 规格

      40μg

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    产品概述
    Specificity This Antibody recognizes VSV-G tagged fusion proteins.
    Host Species Rabbit
    Immunogen VSV-G epitope tag peptide YTDIEMNRLGK conjugated to KLH
    Conjugate Unconjugated


    应用指导
    Working concentrations for specific applications should be determined by the investigator.Appropriate concentrations will be affected by severalfactors, including secondary antibody affinity, antigen concentration,sensitivity of detection method, temperature, and length of incubations,etc.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
    Application Recommended Usage
    ELISA 0.05-0.2 µg/ml
    Western Blot 0.1-1.0 µg/ml
    Western Blot 1:100-1:1,000
    Immunoprecipitation (IP) 2-10 µg/mg of lysate
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    产品属性
    Form Lyophilized
    Storage Buffer Lyophilized in PBS, pH7.4, containing 0.02% sodium azide
    Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 1 mg/ml.
    Storage Instructions The antibody is stable for 2-3 weeks if stored at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles.
    Purification Affinity chromatography
    Isotype Rabbit IgG
    Clonality Polyclonal
    Clone ID Not applicable
    Note Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage.Before use, centrifuge the vial briefly to bring down any liquid in the cap.

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    图标文献和实验
    相关实验
    • Generation and Selection of Rabbit Antibody Libraries by Phage Display

      The rabbit antibody repertoire is an exceptional source for both polyclonal antibodies (pAbs) and monoclonal antibodies (mAbs) that combine high specificity with high avidity and affinity, respectively. In contrast to rabbit pAbs

    • Generation of Rabbit Immune Libraries

      gene that is preferentially used, and the diversity of the antibody repertoire being mainly generated by gene conversion and hypermutation, which facilitates the molecular cloning of the rabbit immune repertoire. In this chapter, the general strategy

    • Testing Rabbit Bleeds By Elisa

      at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody

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