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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
广州市左克生物
- 库存:
充足
- 保存条件:
4ºC
- 规格:
40μg
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| Specificity | This Antibody recognizes VSV-G tagged fusion proteins. |
| Host Species | Rabbit |
| Immunogen | VSV-G epitope tag peptide YTDIEMNRLGK conjugated to KLH |
| Conjugate | Unconjugated |
| Working concentrations for specific applications should be determined by the investigator.Appropriate concentrations will be affected by severalfactors, including secondary antibody affinity, antigen concentration,sensitivity of detection method, temperature, and length of incubations,etc.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product. | |||||||||||
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| Form | Lyophilized |
| Storage Buffer | Lyophilized in PBS, pH7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 1 mg/ml. |
| Storage Instructions | The antibody is stable for 2-3 weeks if stored at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles. |
| Purification | Affinity chromatography |
| Isotype | Rabbit IgG |
| Clonality | Polyclonal |
| Clone ID | Not applicable |
| Note | Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage.Before use, centrifuge the vial briefly to bring down any liquid in the cap. |
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文献和实验Generation and Selection of Rabbit Antibody Libraries by Phage Display
The rabbit antibody repertoire is an exceptional source for both polyclonal antibodies (pAbs) and monoclonal antibodies (mAbs) that combine high specificity with high avidity and affinity, respectively. In contrast to rabbit pAbs
Generation of Rabbit Immune Libraries
gene that is preferentially used, and the diversity of the antibody repertoire being mainly generated by gene conversion and hypermutation, which facilitates the molecular cloning of the rabbit immune repertoire. In this chapter, the general strategy
Testing Rabbit Bleeds By Elisa
at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody
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