- ￥2500
- GenScript
- A00186-100
- 2026年05月27日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
广州市左克生物
- 库存：
充足
- 保存条件：
4ºC
- 规格：
100μg
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| Specificity | THE™ His Tag Antibody, mAb, Mouse recognizes His tags localized at the C-terminal, N-terminal, and internal region of fusion proteins. |
| Host Species | Mouse |
| Immunogen | A synthetic peptide HHHHHH coupled to KLH |
| Conjugate | Unconjugated |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. | |||||||||||||||
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| Form | Lyophilized |
| Storage Buffer | Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution | Reconstitute the lyophilized powder with sterile water (or equivalent) to a final concentration of 0.5 mg/mL. |
| Storage Instructions | The product remains stable for 1 year at -20°C from the date of receipt. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C.Avoid repeated freezing and thawing cycles. |
| Purification | Purified by Protein A affinity chromatography. |
| Isotype | Mouse IgG1,κ |
| Clonality | Monoclonal |
| Clone ID | 6G2A9 |
| Note | GenScript can offer this product according to your requirement, including product size, buffer components, etc. |
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文献和实验1. Materials(1) Antibody 7E3, 2L sup grown in flasks, frozen and thawed overnight.(2) BioRad Affi-Gel Protein A MAPS II Buffers cat. #1530-6160 ($161.00)(3) 50 mM Tris pH7.8.(4) 40 g ammonium sulfate for every 100 ml Sup.(5) 4L 100 mM Tris/pH
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
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