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- 保存条件:
2-8°C
- 保质期:
6个月
- 库存:
100
- 供应商:
百欧泰
- 规格:
10ml
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文献和实验tjmedwnn:请问25uM的氯喹是这样配的吗????大家好,我做质粒转染,说明书上说转染前要加25uM的氯喹。我买的氯喹分子量是515.86,请问大家如果换算成质量这怎么计算啊,是不是25/515.86=0.048μg然后称取0.048μg加进去啊,请大家多教教我,谢谢!丁香园网友szcsyan认为:一般配成stock,浓度高一点。比如25mM。用的时候再稀释。看你买的是多重的。比如X(mg)->; X/515.86 (毫摩)容在Vμl的DMSO(一般用DMSO溶)= 25mM
A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
Purification of U Small Nuclear Ribonucleoprotein Particles
cell nuclear extracts (i.e., at about 150 mM salt concentration), the spliceosomal snRNPs are organized in three RNP forms: 12S U1, 17S U2, and 25S [U4/U6.U5] trisnRNP. These snRNP complexes may be considered as functional subunits of the spliceosome.
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