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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C; keep this chemical dry and dark.
- 保质期:
见COA
- 库存:
大量
- 供应商:
北京辰辉创聚生物技术有限公司
- CAS号:
65851-39-0
- 规格:
1mg;100mg;1g;Bulk

| 产品信息
品名:NebuChem™ 5-Chloro-8-hydroxyquinoline β-D-glucuronide
货号:NBC-243260
品牌:Nebulabio
规格:1mg;100mg;1g;Bulk
别名:5-Chloro-8-quinolinyl β-D-glucopyranosiduronic acid
| 产品属性
CAS号:65851-39-0
分子式:C15H14ClNO7
分子量:355.73
溶解度:DMSO, H2O, Methanol
外观:See COA
储存条件:Keep dry, cool and dark.
For Research Use Only!
To get more information, please contact us freely.
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文献和实验Histochemical detection重组质体的检定
操作原理与colony hybridization 相同,但是将滤膜移置于含有IPTG 及5-bromo-4-chloro-3-indolyl β-D-glucuronide (X-Gluc) 的培养基。 X-Gluc 可被GUS作用而释出蓝色的产物,若转型株所带的质体接有gus 基因的重组质体,则应可在IPTG 的诱导条件下表现出蛋白质,若此重组蛋白质具有GUS 活性,可将进入菌体的X-Gluc 分解而使菌落呈蓝色。 仪器用具: 平端镊子 药品试剂: Nitrocellulose
P-Glucuronidase Enzyme Histochemistry on Semithin Sections of Plastic-Embedded Arabidopsis Explants
expressing the reporter gene within the cellular context of the explant or tissue. A qualitative hrstochemical assay to measure GUS activity is based on the substrate 5-bromo-4-chloro-3-mdolyLl3-o-glucuronide (X-Glut) ( 1 ). The GUS enzyme hydrolyzes X-Glut
and section tissues. Additionally, β-Galactosidase (β-gal) is a popular reporter for detecting the expression of endogenous or exogenous genes. We reveal that 6-chloro-3-indoxyl-β-d -galactopyranoside (S-gal) is a more sensitive substrate for β-gal activity
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