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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海酶研生物科技有限公司
- 英文名:
FM-acf (Fibroblast Medium-animal component free)
- 规格:
500ml/T
|
货号 |
2321 |
|
产地 |
美国 |
|
缩写 |
FM-acf |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
基础液4度,因子-20度 |
|
运输 |
胶冰 |
无动物成分成纤维细胞培养基是为正常人类成纤维细胞体外培养设计的适于其生长的培养基。它是经灭菌的液体培养基,包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质。该培养基缓冲体系为HEPES和重碳酸盐,在含5%CO2的细胞培养箱中平衡后pH值为7.4。该培养基的配方能够选择性的促进正常人类成纤维细胞体外培养中的增殖和生长,并为其达到理想营养平衡状态提供数量上和质量上的保证。
无动物成纤维细胞培养基包含500 ml基础培养基,5ml成纤维细胞生长添加物(FGS-acf,目录编号2372)和5 ml青霉素/链霉素溶液(P/S,目录编号0503)
33.) Yue X, Xu L, Zhu M, Zhang R, Liu C. (2013) "Over-expression of Nerve Growth Factor-α in human cholangiocarcinoma QBC939 cells promote tumor progression."?PLoS One. 8: e62024.
34.) Crowe A, Jackaman C, Beddoes KM, Ricciardo B, Nelson DJ. (2013) "Rapid copper acquisition by developing murine mesothelioma: decreasing bioavailable copper slows tumor growth, normalizes vessels and promotes T cell infiltration." PLoS One. 8: e73684.
35.) Zhao XC, Dou GR, Wang L, Liang L, Tian DM, Cao XL, Qin HY, Wang CM, Zhang P, Han H. (2013) "Inhibition of Tumor Angiogenesis and Tumor Growth by the DSL Domain of Human Delta-Like 1 Targeted to Vascular Endothelial Cells."?Neoplasia. 15 :815-25
36.) Lachance PA, Hazen A, Sevick-Muraca EM. (2013) "Lymphatic Vascular Response to Acute Inflammation."?PLoS One. 8: e76078.
37.) Aisha AF, Ismail Z, Abu-Salah KM, Siddiqui JM, Ghafar G, Abdul Majid AM. (2013) "Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice"?BMC Complement Altern Med. 13: 168.?
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文献和实验
33.) Yue X, Xu L, Zhu M, Zhang R, Liu C. (2013) "Over-expression of Nerve Growth Factor-α in human cholangiocarcinoma QBC939 cells promote tumor progression."?PLoS One. 8: e62024.
34.) Crowe A, Jackaman C, Beddoes KM, Ricciardo B, Nelson DJ. (2013) "Rapid copper acquisition by developing murine mesothelioma: decreasing bioavailable copper slows tumor growth, normalizes vessels and promotes T cell infiltration." PLoS One. 8: e73684.
35.) Zhao XC, Dou GR, Wang L, Liang L, Tian DM, Cao XL, Qin HY, Wang CM, Zhang P, Han H. (2013) "Inhibition of Tumor Angiogenesis and Tumor Growth by the DSL Domain of Human Delta-Like 1 Targeted to Vascular Endothelial Cells."?Neoplasia. 15 :815-25
36.) Lachance PA, Hazen A, Sevick-Muraca EM. (2013) "Lymphatic Vascular Response to Acute Inflammation."?PLoS One. 8: e76078.
37.) Aisha AF, Ismail Z, Abu-Salah KM, Siddiqui JM, Ghafar G, Abdul Majid AM. (2013) "Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice"?BMC Complement Altern Med. 13: 168.?
Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
embryonic fibroblasts (MEFs) or human feeder cells. In this protocol, we describe the culture of hESCs in feeder-free conditions on Matrigel with MEF-conditioned medium. This protocol can be used for applications such as genetic modification of hESCs
R&D Systems Animal Free Proteins
在不含动物成分的环境下生产的优质产品 √ 使用不含动物成分的专门设备生产 √ 现有产品包括细胞因子、生长因子及其他 √ 在我们专门的不含动物成分的工厂中制造 √ 在生产过程中未暴露于动物来源的成分 √ 生物活性与标准实验室条件下生产的蛋白相当 √ 内毒素水平低 √ 科研或生物生产的绝佳选择 √ 可提供更多证明文件 R&D Systems的Animal-Free制造工序
指完全不含微生物,特别是不含病原菌的动物。但一般并不排除病毒。这种动物用剖腹手术自母体中取出,在无菌室中以无菌饲料喂养;通过无菌的雌雄动物交配也可获得。这种动物用来系统调查各种微生物对动物的影响。此外,把防止特定病原体感染的条件称为无特定病原( SPF, specific pathogenfree),把只能使特定细菌寄生或共生的条件称作择生生物( gnotobiotic),这种装置则称为择生装置( gnotobiotron)。与此相应,尽可能在清洁条件下饲养,但未作上述考虑而饲养
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