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- Sciencell
- 2301
- 美国
- 2025年08月01日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
上海酶研生物科技有限公司
- 英文名：
FM (Fibroblast Medium)
- 规格：
500ml/T
|
货号 |
2301 |
|
产地 |
美国 |
|
缩写 |
FM |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
4度，-20度 |
|
运输 |
胶冰 |
成纤维细胞培养基是为正常人类成纤维细胞体外培养设计的适于其生长的培养基。它是经灭菌的液体培养基，包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质和低浓度胎牛血清(2%)。该培养基缓冲体系为HEPES和重碳酸盐，在含5%CO2的细胞培养箱中平衡后pH值为7.4。该培养基的配方能够选择性的促进正常人类成纤维细胞体外培养中的增殖和生长，并为其达到理想营养平衡状态提供数量上和质量上的保证。
成纤维细胞培养基包含500 ml基础培养基，10ml胎牛血清(FBS,目录编号0010)，5ml成纤维细胞生长添加物(FGS,目录编号2352)和5 ml青霉素/链霉素溶液(P/S,目录编号0503)
1.) Holton SE, Bergamaschi A, Katzenellenbogen BS, Bhargava R. (2014) 'Integration of Molecular Profiling and Chemical Imaging to Elucidate Fibroblast-Microenvironment Impact on Cancer Cell Phenotype and Endocrine Resistance in Breast Cancer.'
2.) Holton SE, Bergamaschi A, Katzenellenbogen BS, Bhargava R. (2014) "Integration of Molecular Profiling and Chemical Imaging to Elucidate Fibroblast-Microenvironment Impact on Cancer Cell Phenotype and Endocrine Resistance in Breast Cancer." PloS one 9: e96878.
3.) Li F, Weir MD, Chen J, Xu HHK. (2013) "Comparison of quaternary ammonium-containing with nano-silver-containing adhesive in antibacterial properties and cytotoxicity."Dent Mater. 29: 450-61.
4.) Williams M, Villarreal A, Bozhilov K, Lin S, Talbot P. (2013) "Metal and silicate particles including nanoparticles are present in electronic cigarette cartomizer fluid and aerosol." PLoS One. 8: e57987.
5.) Zhang K, Cheng L, Imazato S, Antonucci JM, Lin NJ, Lin-Gibson S, Bai Y, Xu HHK. (2013) "Effects of dual antibacterial agents MDPB and nano-silver in primer on microcosm biofilm, cytotoxicity and dentin bond properties." J Dent. 41: 464-74.
6.) Cavet ME, Volhejn S, Harrington KL, Zhang JZ. (2013) "Anti-allergic effects of mapracorat, a novel selective glucocorticoid receptor agonist, in human conjunctival fibroblasts and epithelial cells." Mol Vis. 19: 1515-25.
7.) Gong Y, Scott E, Lu R, Xu Y, Oh WK, Yu Q (2013) 'TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression.'
8.) Gong Y, Scott E, Lu R, Xu Y, Oh WK, Yu Q (2013) "TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression." PloS one. 8: e77366.
9.) Wang K, Zhang S, Weber J, Baxter D, Galas DJ. (2010) 'Export of microRNAs and microRNA-protective protein by mammalian cells.'
10.) Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC. (2010) 'B?type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts.'
11.) Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC. (2010) "B?type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts." J of cell physiol. 225: 251-55.
12.) Wang K, Zhang S, Weber J, Baxter D, Galas DJ. (2010) "Export of microRNAs and microRNA-protective protein by mammalian cells." Nucleic Acids Res. 38:7248-59.
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文献和实验
1.) Holton SE, Bergamaschi A, Katzenellenbogen BS, Bhargava R. (2014) 'Integration of Molecular Profiling and Chemical Imaging to Elucidate Fibroblast-Microenvironment Impact on Cancer Cell Phenotype and Endocrine Resistance in Breast Cancer.'
2.) Holton SE, Bergamaschi A, Katzenellenbogen BS, Bhargava R. (2014) "Integration of Molecular Profiling and Chemical Imaging to Elucidate Fibroblast-Microenvironment Impact on Cancer Cell Phenotype and Endocrine Resistance in Breast Cancer." PloS one 9: e96878.
3.) Li F, Weir MD, Chen J, Xu HHK. (2013) "Comparison of quaternary ammonium-containing with nano-silver-containing adhesive in antibacterial properties and cytotoxicity."Dent Mater. 29: 450-61.
4.) Williams M, Villarreal A, Bozhilov K, Lin S, Talbot P. (2013) "Metal and silicate particles including nanoparticles are present in electronic cigarette cartomizer fluid and aerosol." PLoS One. 8: e57987.
5.) Zhang K, Cheng L, Imazato S, Antonucci JM, Lin NJ, Lin-Gibson S, Bai Y, Xu HHK. (2013) "Effects of dual antibacterial agents MDPB and nano-silver in primer on microcosm biofilm, cytotoxicity and dentin bond properties." J Dent. 41: 464-74.
6.) Cavet ME, Volhejn S, Harrington KL, Zhang JZ. (2013) "Anti-allergic effects of mapracorat, a novel selective glucocorticoid receptor agonist, in human conjunctival fibroblasts and epithelial cells." Mol Vis. 19: 1515-25.
7.) Gong Y, Scott E, Lu R, Xu Y, Oh WK, Yu Q (2013) 'TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression.'
8.) Gong Y, Scott E, Lu R, Xu Y, Oh WK, Yu Q (2013) "TIMP-1 Promotes Accumulation of Cancer Associated Fibroblasts and Cancer Progression." PloS one. 8: e77366.
9.) Wang K, Zhang S, Weber J, Baxter D, Galas DJ. (2010) 'Export of microRNAs and microRNA-protective protein by mammalian cells.'
10.) Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC. (2010) 'B?type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts.'
11.) Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC. (2010) "B?type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts." J of cell physiol. 225: 251-55.
12.) Wang K, Zhang S, Weber J, Baxter D, Galas DJ. (2010) "Export of microRNAs and microRNA-protective protein by mammalian cells." Nucleic Acids Res. 38:7248-59.
The Fibroblast-Populated Collagen Lattice: A Model of Fibroblast Collagen Interactions in Repair
and collagen. A better understanding of those interactions may lead to a more rapid closure of wounds and will reduce the frequency of abnormal scarring. An approach to better understand that process is through in vitro models. One such model is the fibroblast
In Vitro Matrigel Fibroblast Invasion Assay
with a thin layer of matrigel. On top of this matrigel fibroblast-like synoviocytes are seeded at a density of 100,000 cells per milliliter. The cells are cultured in serum free medium in the inner compartment inside the transwell. To the outer compartment
一般是指包围在生活系统的外围，并与其表面保持直接接触的物质，也可以说就是成为生活系统的生活场所的物质。生物，作为其所有物质代谢的结果，是与介质间进行物质交换的。所有的细胞，其活动场所的介质都是液体，此特称为液体介质。陆地生物，作为其个体来说，一般是以空气为介质的。土壤和泥沙应看作是地下生物的介质。在多细胞生物的驱体中，作为构成细胞的直接介质的体液是很发达的，特别是在高等动物中，这种介质，也就是说内部介质（=内部环境）必须从外部介质获得其独立性和稳定性（体内平衡 homeostasis
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