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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海酶研生物科技有限公司
- 英文名:
EpiCM-a (Epithelial Cell Medium-animal)
- 规格:
500ml/T
|
货号 |
4131 |
|
产地 |
美国 |
|
缩写 |
EpiCM-a |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
4度,-20度 |
|
运输 |
胶冰 |
动物上皮细胞培养基是为动物上皮细胞体外培养设计的适于其生长的培养基。培养基是无菌的、液体培养基,包含必需和非必需氨基、维生素、有机和无机化合物、激素、生长因子、微量矿物质、低浓度胎牛血清(2%)。该培养基含碳酸氢盐缓冲体系,在5%二氧化碳/95%空气培养箱中平衡时PH值为7.4。该培养基在数量上和质量上都保证理想的营养平衡状态,选择性促进体外动物上皮细胞的生长。
动物上皮细胞培养基包含500 ml基础培养基,5 ml上皮细胞无动物成分生长添加物,(EpiCGS-a,目录编号4182)和5 ml青霉素/链霉素溶液(P/S,目录编号0503) 注:由于生长因子中的BPE,会导致脂蛋白形成沉淀。
17.) Guo W, Ding J, Zhang A, Dai W, Liu S, Diao Z, Wang L, Han X, Liu W. (2014) "The Inhibitory Effect of Quercetin on Asymmetric Dimethylarginine-Induced Apoptosis Is Mediated by the Endoplasmic Reticulum Stress Pathway in Glomerular Endothelial Cells."?Int j mol sci. 15: 484-503.
18.) Lim PJ, Chu JJH. (2014) "A Polarized Cell Model for Chikungunya Virus Infection: Entry and Egress of Virus Occurs at the Apical Domain of Polarized Cells."?PLoSnegl trop dis. 8: e2661.
19.) Liu Q, Hou J, Chen X, Liu G, Zhang D, Sun H, Zhang J. (2014) "P-Glycoprotein Mediated Efflux Limits the Transport of the Novel Anti-Parkinson's Disease Candidate Drug FLZ across the Physiological and PD Pathological In Vitro BBB Models."?PloS one. 9: e102442.
20.) Tong X, Lv P, Mathew AV, Liu D, Niu C, Wang Y, Ji L, Li J, Fu Z, Pan B, Pennathur S, Zheng L, Huang Y. (2014) "The compensatory enrichment of sphingosine-1-phosphate harbored on glycated high-density lipoprotein restores endothelial protective function in type 2 diabetes mellitus."?Cardiovascdiabetol. 13: 82.
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文献和实验
17.) Guo W, Ding J, Zhang A, Dai W, Liu S, Diao Z, Wang L, Han X, Liu W. (2014) "The Inhibitory Effect of Quercetin on Asymmetric Dimethylarginine-Induced Apoptosis Is Mediated by the Endoplasmic Reticulum Stress Pathway in Glomerular Endothelial Cells."?Int j mol sci. 15: 484-503.
18.) Lim PJ, Chu JJH. (2014) "A Polarized Cell Model for Chikungunya Virus Infection: Entry and Egress of Virus Occurs at the Apical Domain of Polarized Cells."?PLoSnegl trop dis. 8: e2661.
19.) Liu Q, Hou J, Chen X, Liu G, Zhang D, Sun H, Zhang J. (2014) "P-Glycoprotein Mediated Efflux Limits the Transport of the Novel Anti-Parkinson's Disease Candidate Drug FLZ across the Physiological and PD Pathological In Vitro BBB Models."?PloS one. 9: e102442.
20.) Tong X, Lv P, Mathew AV, Liu D, Niu C, Wang Y, Ji L, Li J, Fu Z, Pan B, Pennathur S, Zheng L, Huang Y. (2014) "The compensatory enrichment of sphingosine-1-phosphate harbored on glycated high-density lipoprotein restores endothelial protective function in type 2 diabetes mellitus."?Cardiovascdiabetol. 13: 82.
实验材料:1. 动物胎体胃黏膜,胃溃疡或胃癌手术切除的正常胃黏膜组织2. 1%Ⅳ胶原蛋白或1%明胶铺培养瓶,4℃冰箱内过夜,使用前在37℃培养箱内放置2h,然后用培养液浸洗1次。3. 不含Ca2+ 和Mg2+ 的1×PBS,添加200000IU/L青霉素、200mg/L链霉素和200000U/L庆大霉素,pH7.44. 250000U/LⅠ型胶原酶或125000U/LⅠ型胶原酶和0.5%透明质酸酶,DMEM配置5. 解剖剪、解剖镊、眼科剪,眼科镊6. 离心管(15ml、50
实验材料: 1. 动物胎体小肠黏膜或死亡胎儿的小肠黏膜 2. 1%Ⅳ胶原蛋白铺培养瓶,放超净工作台上干燥 3. 不含Ca2+ 和Mg2+ 的1×PBS,添加200000IU/L青霉素、200mg/L链霉素和200000U/L庆大霉素,pH7.4 4. 300000U/LⅪ型胶原酶和0.1g/LⅠ型中性蛋白酶混合消化液,DMEM配置 5. 解剖剪、解剖镊、眼科剪,眼科镊 6. 离心管(15ml、50ml) 实验方法: 1. 取妊娠17-19d
实验材料: 1. 胸腺上皮细胞来源;一般取材小鼠或手术切取的儿童之胸腺; 2. 不含Ca2+ 和Mg2+ 的1×PBS,添加200000IU/L青霉素、200mg/L链霉素,pH7.2; 3. 有血清培养液:可使用RPMI1640培养液,添加10%胎牛血清、谷氨酰胺2mmol/L、丙酮酸钠1mmol/L、非必需氨基酸1mmol/L、2-巯基乙醇(2-ME)5×10-5 mol/L、青霉素100IU/ml、链霉素100μg/ml
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