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- Sciencell
- C-7311
- 美国
- 2025年08月01日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
上海酶研生物科技有限公司
- 英文名：
OEpiCM (Ovarian Epithelial Cell Medium)
- 规格：
500ml/T
|
货号 |
7311 |
|
产地 |
美国 |
|
缩写 |
OEpiCM |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
4度，-20度 |
|
运输 |
胶冰 |
Ovarian Epithelial Cell Medium (OEpiCM) is a complete medium designed for optimal growth of normal human ovarian epithelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors and trace minerals. The medium is serum-free. It is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human ovarian epithelial cells in vitro.
1.) Friedman E. (2013) "Mirk/dyrk1B kinase in ovarian cancer." Int J Mol Sci. 14: 5560
2.) Cha Y, Kim DK, Hyun J, Kim SJ, Park KS. (2013) " TCEA3 binds to TGF-beta receptor I and induces Smad-independent, JNK-dependent apoptosis in ovarian cancer cells." Cellular Signal. 25: 1245-51.
3.) Takahashi Y, Koyanagi T, Suzuki Y, Saga Y, Kanomata N, Moriya T, Suzuki M, Sato Y. (2012) "Vasohibin-2 expressed in human serous ovarian adenocarcinoma accelerates tumor growth by promoting angiogenesis." Mol Cancer Res. 10: 1135-46.
4.) Semaan A, Qazi AM, Seward S, Chamala S, Bryant CS, Kumar S, Morris R, Steffes CP, Bouwman DL, Mukarah AR, Weaver DW, Gruber SA, Batchu RB. (2011) "MicroRNA-101 Inhibits Growth of Epithelial Ovarian Cancer by Relieving Chromatin-Mediated Transcriptional Repression of p21waf1/cip1." Pharm Res. 28: 3079-90.
5.) Guo AM, Liu X, Al-Wahab Z, Maddippati KR, Ali-Fehmi R, Scicli AG, Munkarah AR. (2011) "Role of 12-lipoxygenase in regulation of ovarian cancer cell proliferation and survival." Cancer ChemotherPharmacol.68: 1273-83.
6.) Selvendiran K, Ahmed S, Dayton A, Kuppusamy ML, Tazi M, Bratasz A, Tong L, Rivera BK, Kalai T, Hideg K, Kuppusamy P. (2010) "Safe and targeted anticancer efficacy of a novel class of antioxidant-conjugated difluorodiarylidenylpiperidones: differential cytotoxicity in healthy and cancer cells." Free RadicBiol Med.48: 1228-35.
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文献和实验
1.) Friedman E. (2013) "Mirk/dyrk1B kinase in ovarian cancer." Int J Mol Sci. 14: 5560
2.) Cha Y, Kim DK, Hyun J, Kim SJ, Park KS. (2013) " TCEA3 binds to TGF-beta receptor I and induces Smad-independent, JNK-dependent apoptosis in ovarian cancer cells." Cellular Signal. 25: 1245-51.
3.) Takahashi Y, Koyanagi T, Suzuki Y, Saga Y, Kanomata N, Moriya T, Suzuki M, Sato Y. (2012) "Vasohibin-2 expressed in human serous ovarian adenocarcinoma accelerates tumor growth by promoting angiogenesis." Mol Cancer Res. 10: 1135-46.
4.) Semaan A, Qazi AM, Seward S, Chamala S, Bryant CS, Kumar S, Morris R, Steffes CP, Bouwman DL, Mukarah AR, Weaver DW, Gruber SA, Batchu RB. (2011) "MicroRNA-101 Inhibits Growth of Epithelial Ovarian Cancer by Relieving Chromatin-Mediated Transcriptional Repression of p21waf1/cip1." Pharm Res. 28: 3079-90.
5.) Guo AM, Liu X, Al-Wahab Z, Maddippati KR, Ali-Fehmi R, Scicli AG, Munkarah AR. (2011) "Role of 12-lipoxygenase in regulation of ovarian cancer cell proliferation and survival." Cancer ChemotherPharmacol.68: 1273-83.
6.) Selvendiran K, Ahmed S, Dayton A, Kuppusamy ML, Tazi M, Bratasz A, Tong L, Rivera BK, Kalai T, Hideg K, Kuppusamy P. (2010) "Safe and targeted anticancer efficacy of a novel class of antioxidant-conjugated difluorodiarylidenylpiperidones: differential cytotoxicity in healthy and cancer cells." Free RadicBiol Med.48: 1228-35.
胎牛血清是细胞培养常用的添加物， 目前享有盛誉的Life等国际品牌的最好胎牛血清价格飞涨，500ML从原来的3000-4000元涨到现在7000-8000元，给很多科研单位或机构造成了巨大的压力，不管是科研单位还是经销商都费劲心机储备高质量的国际进口血清。然而前期储备的较低价格的进口血清总有用完的一天， 因此国内相关单位非常关注寻求新型的细胞培养基。 但并不是所有的细胞培养需要最高质量的胎牛血清， 而且市场上有些培养基完全可以替代昂贵的Life等国际品牌血清。 笔者对哺乳细胞培养过程中培养
和数量充足，干细胞/原代细胞上清可在提取前采用超滤管（100KD）浓缩 3~5 倍后再提取。 3、使用EPF柱时堵塞如何处理？ 答：过滤柱的孔径在 220nm 左右。如果出现堵塞情况，可以将柱子旋转 180 度再次离心。若上室还有残留液体，需要使用新的 EPF 柱，该柱子可以单独购买（货号 UR90102）。 4、沉淀法的具体原理是什么？ 答：将高亲水性聚合物（聚乙二醇等）添加到含外泌体的溶液中后，外泌体周围的水分子被聚合物束缚，降低了外泌体的溶解度并诱导其随后的沉淀，使外泌体在低速离心下可以很容易
的培养基，让研究人员能完全掌控实验状况和时机，而价格也相当有吸引力。 近几年，大量研究显示新鲜制备的原代细胞比细胞系更能准确地模拟体内环境。而且3D细胞培养又比2D细胞培养更能体现体内的环境。因此，用原代细胞构建的3D培养是模拟体内复杂生物过程的最准确方法。 3D体外模型的建立对细胞培养基 提出了更严格的要求。首先，该培养基必须促进细胞终端分化，细胞分层并形成多层的结构。然而，它又必须能维持基底层的增殖细胞，这样才能持续供应模型所需的未分化细胞。这种矛盾的需求是传统的培养
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