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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海酶研生物科技有限公司
- 英文名:
PM (Pericyte Medium)
- 规格:
500ml/T
|
货号 |
1201 |
|
产地 |
美国 |
|
缩写 |
PM |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
4度,-20度 |
|
运输 |
胶冰 |
周边细胞培养基是为正常人类血管周边细胞体外培养设计的适于其生长的培养基。是经灭菌的液体培养基,包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质和低浓度胎牛血清(2%)。该培养基缓冲体系为重碳酸盐,在含5%CO2的细胞培养箱中平衡后pH值为7.4。该培养基的配方能够选择性的促进正常人类周边细胞体外培养中的增殖和生长,并为其达到理想营养平衡状态提供数量上和质量上的保证。
周边细胞培养基包含500 ml基础培养基,10ml胎牛血清(FBS,目录编号0010),5ml周边细胞生长添加物(PGS,目录编号1252),和5 ml青霉素/链霉素溶液(P/S,目录编号0503)
1.) You WK, Yotsumoto F, Sakimura K, Adams RH, Stallcup WB. (2014) 'NG2 proteoglycan promotes tumor vascularization via integrin-dependent effects on pericyte function.'
2.) You WK, Yotsumoto F, Sakimura K, Adams RH, Stallcup WB. (2014) "NG2 proteoglycan promotes tumor vascularization via integrin-dependent effects on pericyte function." Angiogenesis. 17: 61-76.
3.) Tigges U, Boroujerdi A, Welser-Alves JV, Milner R. (2013) "TNF-α promotes cerebral pericyte remodeling in vitro, via a switch from 1 to 2 integrins." J Neuroinflammation. 10: 33.
4.) Mandrup OA, Friis NA, Lykkemark S, Just J, Kristensen P. (2013) "A Novel Heavy Domain Antibody Library with Functionally Optimized Complementarity Determining Regions." PLoS One. 8: e76834.
5.) Cho MK, Sun ES, Kim YH. (2013) 'Zinc-Triggered Induction of Tissue Plasminogen Activator and Plasminogen in Endothelial Cells and Pericytes.' Expneurobiol.
6.) Cho MK, Sun ES, Kim YH. (2013) "Zinc-Triggered Induction of Tissue Plasminogen Activator and Plasminogen in Endothelial Cells and Pericytes." Expneurobiol. 22: 315-21.
7.) Tigges U, Welser-Alves JV, Boroujerdi A, Milner R. (2012) 'A novel and simple method for culturing pericytes from mouse brain.'
8.) Tigges U, Welser-Alves JV, Boroujerdi A, Milner R. (2012) "A novel and simple method for culturing pericytes from mouse brain." Microvasc res. 84: 74-80.
9.) Bagley RG, Weber W, Rouleau C, Teicher BA. (2005) "Pericytes and endothelial precursor cells: cellular interactions and contributions to malignancy." Cancer Res. 65: 9741-50.
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文献和实验
1.) You WK, Yotsumoto F, Sakimura K, Adams RH, Stallcup WB. (2014) 'NG2 proteoglycan promotes tumor vascularization via integrin-dependent effects on pericyte function.'
2.) You WK, Yotsumoto F, Sakimura K, Adams RH, Stallcup WB. (2014) "NG2 proteoglycan promotes tumor vascularization via integrin-dependent effects on pericyte function." Angiogenesis. 17: 61-76.
3.) Tigges U, Boroujerdi A, Welser-Alves JV, Milner R. (2013) "TNF-α promotes cerebral pericyte remodeling in vitro, via a switch from 1 to 2 integrins." J Neuroinflammation. 10: 33.
4.) Mandrup OA, Friis NA, Lykkemark S, Just J, Kristensen P. (2013) "A Novel Heavy Domain Antibody Library with Functionally Optimized Complementarity Determining Regions." PLoS One. 8: e76834.
5.) Cho MK, Sun ES, Kim YH. (2013) 'Zinc-Triggered Induction of Tissue Plasminogen Activator and Plasminogen in Endothelial Cells and Pericytes.' Expneurobiol.
6.) Cho MK, Sun ES, Kim YH. (2013) "Zinc-Triggered Induction of Tissue Plasminogen Activator and Plasminogen in Endothelial Cells and Pericytes." Expneurobiol. 22: 315-21.
7.) Tigges U, Welser-Alves JV, Boroujerdi A, Milner R. (2012) 'A novel and simple method for culturing pericytes from mouse brain.'
8.) Tigges U, Welser-Alves JV, Boroujerdi A, Milner R. (2012) "A novel and simple method for culturing pericytes from mouse brain." Microvasc res. 84: 74-80.
9.) Bagley RG, Weber W, Rouleau C, Teicher BA. (2005) "Pericytes and endothelial precursor cells: cellular interactions and contributions to malignancy." Cancer Res. 65: 9741-50.
基的选择总结如下心得,以供大家参考: 1) 肿瘤细胞系及悬浮细胞的培养基选择 肿瘤细胞系是最容易饲养的细胞,对胎牛血清要求不高,甚至用新生牛血清就可以了,没有必要用高质量的Life或澳洲胎牛血清。比如,四季青、吉泰、WHB等国产牌子的血清完全可以胜任。 对于CHO悬浮细胞,甚至不用添加血清,选择将DMEM作为基本培养基,并添加培养细菌用的有酵母抽提物或植物抽提物就完全可以胜任,超便宜,可以配500 ML不会超过100元。 2) 原代哺乳细胞培养基的选择 对于需要扩增的原代哺乳
的培养基,让研究人员能完全掌控实验状况和时机,而价格也相当有吸引力。 近几年,大量研究显示新鲜制备的原代细胞比细胞系更能准确地模拟体内环境。而且3D细胞培养又比2D细胞培养更能体现体内的环境。因此,用原代细胞构建的3D培养是模拟体内复杂生物过程的最准确方法。 3D体外模型的建立对细胞培养基 提出了更严格的要求。首先,该培养基必须促进细胞终端分化,细胞分层并形成多层的结构。然而,它又必须能维持基底层的增殖细胞,这样才能持续供应模型所需的未分化细胞。这种矛盾的需求是传统的培养
xCELLigence实时细胞分析系统主要应用(一):神经毒性 特征性研究: xCELLigence系统实时检测神经细胞死亡 关键词: 神经细胞死亡、xCELLigence系统、原代皮质神经元细胞、 HT-22 细胞、细胞阻抗、实时测定 作者: Sebastian Diemert、Julia Grohm、Svenja Tobaben、Amalia Dolga、Carsten Culmsee 德国 Philipps
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