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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海酶研生物科技有限公司
- 英文名:
ECM (Endothelial Cell Medium)
- 规格:
500ml/T
|
货号 |
1001 |
|
产地 |
北美 |
|
缩写 |
ECM |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
4度,-20度 |
|
运输 |
胶冰 |
Endothelial Cell Medium (ECM), when used with Endothelial Cell Growth Supplement (ECGS, Cat #1052) and 25 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal endothelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal endothelial cells in vitro.
8.) Umar MI, Asmawi MZ, Sadikun A, Majid AMSA, Al-Suede FSR, Hassan LEA, Altaf R, Ahamed MBK. (2014) 'Ethyl-p-methoxycinnamate isolated from kaempferiagalanga inhibits inflammation by suppressing interleukin-1, tumor necrosis factor-?, and angiogenesis by blocking endothelial functions.'
9.) Tong X, Lv P, Mathew AV, Liu D, Niu C, Wang Y, Ji L, Li J, Fu Z, Pan B, Pennathur S, Zheng L, Huang Y. (2014) 'The compensatory enrichment of sphingosine-1-phosphate harbored on glycated high-density lipoprotein restores endothelial protective function in type 2 diabetes mellitus.'
10.) Liu Q, Hou J, Chen X, Liu G, Zhang D, Sun H, Zhang J. (2014) 'P-Glycoprotein Mediated Efflux Limits the Transport of the Novel Anti-Parkinson's Disease Candidate Drug FLZ across the Physiological and PD Pathological In Vitro BBB Models.'
11.) Lim PJ, Chu JJH. (2014) 'A Polarized Cell Model for Chikungunya Virus Infection: Entry and Egress of Virus Occurs at the Apical Domain of Polarized Cells.'
12.) Guo W, Ding J, Zhang A, Dai W, Liu S, Diao Z, Wang L, Han X, Liu W. (2014) 'The Inhibitory Effect of Quercetin on Asymmetric Dimethylarginine-Induced Apoptosis Is Mediated by the Endoplasmic Reticulum Stress Pathway in Glomerular Endothelial Cells.'
13.) AbuBakar S, Shu MH, Johari J, Wong PF. (2014) 'Senescence Affects Endothelial Cells Susceptibility to Dengue Virus Infection.'
14.) Adams Y, Kuhnrae P, Higgins MK, Ghumra A, Rowe JA. (2014) 'Rosetting Plasmodium falciparum-Infected Erythrocytes Bind to Human Brain Microvascular Endothelial Cells In Vitro, Demonstrating a Dual Adhesion Phenotype Mediated by Distinct P. falciparum Erythrocyte Membrane Protein 1 Domains.'
15.) AbuBakar S, Shu MH, Johari J, Wong PF. (2014) "Senescence Affects Endothelial Cells Susceptibility to Dengue Virus Infection."?Int j med sci. 11: 538.
16.) Adams Y, Kuhnrae P, Higgins MK, Ghumra A, Rowe JA. (2014) "Rosetting Plasmodium falciparum-Infected Erythrocytes Bind to Human Brain Microvascular Endothelial Cells In Vitro, Demonstrating a Dual Adhesion Phenotype Mediated by Distinct P. falciparum Erythrocyte Membrane Protein 1 Domains."?Infect immune. 82: 949-59.
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文献和实验8.) Umar MI, Asmawi MZ, Sadikun A, Majid AMSA, Al-Suede FSR, Hassan LEA, Altaf R, Ahamed MBK. (2014) 'Ethyl-p-methoxycinnamate isolated from kaempferiagalanga inhibits inflammation by suppressing interleukin-1, tumor necrosis factor-?, and angiogenesis by blocking endothelial functions.'
9.) Tong X, Lv P, Mathew AV, Liu D, Niu C, Wang Y, Ji L, Li J, Fu Z, Pan B, Pennathur S, Zheng L, Huang Y. (2014) 'The compensatory enrichment of sphingosine-1-phosphate harbored on glycated high-density lipoprotein restores endothelial protective function in type 2 diabetes mellitus.'
10.) Liu Q, Hou J, Chen X, Liu G, Zhang D, Sun H, Zhang J. (2014) 'P-Glycoprotein Mediated Efflux Limits the Transport of the Novel Anti-Parkinson's Disease Candidate Drug FLZ across the Physiological and PD Pathological In Vitro BBB Models.'
11.) Lim PJ, Chu JJH. (2014) 'A Polarized Cell Model for Chikungunya Virus Infection: Entry and Egress of Virus Occurs at the Apical Domain of Polarized Cells.'
12.) Guo W, Ding J, Zhang A, Dai W, Liu S, Diao Z, Wang L, Han X, Liu W. (2014) 'The Inhibitory Effect of Quercetin on Asymmetric Dimethylarginine-Induced Apoptosis Is Mediated by the Endoplasmic Reticulum Stress Pathway in Glomerular Endothelial Cells.'
13.) AbuBakar S, Shu MH, Johari J, Wong PF. (2014) 'Senescence Affects Endothelial Cells Susceptibility to Dengue Virus Infection.'
14.) Adams Y, Kuhnrae P, Higgins MK, Ghumra A, Rowe JA. (2014) 'Rosetting Plasmodium falciparum-Infected Erythrocytes Bind to Human Brain Microvascular Endothelial Cells In Vitro, Demonstrating a Dual Adhesion Phenotype Mediated by Distinct P. falciparum Erythrocyte Membrane Protein 1 Domains.'
15.) AbuBakar S, Shu MH, Johari J, Wong PF. (2014) "Senescence Affects Endothelial Cells Susceptibility to Dengue Virus Infection."?Int j med sci. 11: 538.
16.) Adams Y, Kuhnrae P, Higgins MK, Ghumra A, Rowe JA. (2014) "Rosetting Plasmodium falciparum-Infected Erythrocytes Bind to Human Brain Microvascular Endothelial Cells In Vitro, Demonstrating a Dual Adhesion Phenotype Mediated by Distinct P. falciparum Erythrocyte Membrane Protein 1 Domains."?Infect immune. 82: 949-59.
正常人脐静脉原代内皮细胞培养一、实验试剂1、培养基: PriCells Medium + 10% FBS + 1% P/S + PriCells Supplem e nt2、冻存液: PriCells Medium + 20% FBS + 10% DMSO3、洗涤液: 1 × PBS (pH 7.4 ) + 1% P/S4、染色液: 0.4% Trypan Blue5、消化液: PriCells Isolation of Primary Cell Kit6、检测试剂:抗人
细胞培养的基本步骤都有哪些?复苏、传代、冻存的基本操作是什么样的?如果你刚刚开始细胞培养实验,想要建立稳定的细胞培养流程,快来听Dr. 赛的讲解吧。
实验材料: 1. 人或动物的肺部毛细血管 2. PBS,含青霉素100U/ml和链霉素100μg/ml 3. 3mg/ml中性蛋白酶、0.25%胰蛋白酶 4. 眼科剪,眼科镊 5. 20μm尼龙筛、100μm尼龙筛 6. 离心管(15ml、50ml) 实验方法: 1. 将动物麻醉后开胸取
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