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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海酶研生物科技有限公司
- 英文名:
SerCM(Sertoli Cell Medium)
- 规格:
500ml/T
|
货号 |
4521 |
|
产地 |
美国 |
|
缩写 |
SerCM |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
4度,-20度 |
|
运输 |
胶冰 |
Sertoli Cell Medium (SerCM) is a complete medium designed for optimal growth of normal human sertoli cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human sertoli cells in vitro.
31.) Guo W, Zhang D, Wang X, Kong W, Zhang Q, Zhang Y, Liu W. (2013) "Actin cytoskeleton modulates ADMA-induced NF-kappaB nuclear translocation and ICAM-1 expression in endothelial cells."?Med Sci Monit. 17: BR242
32.) Tong X, Peng H, Liu D, Ji L, Niu C, Ren J, Pan B, Hu J, Zheng L, Huang Y. (2013) "High-density lipoprotein of patients with Type 2 Diabetes Mellitus upregulates cyclooxgenase-2 expression and prostacyclin I-2 release in endothelial cells: relationship with HDL-associated sphingosine-1-phosphate."?Cardiovasc Diabetol. 12: 27.
33.) Yue X, Xu L, Zhu M, Zhang R, Liu C. (2013) "Over-expression of Nerve Growth Factor-α in human cholangiocarcinoma QBC939 cells promote tumor progression."?PLoS One. 8: e62024.
34.) Crowe A, Jackaman C, Beddoes KM, Ricciardo B, Nelson DJ. (2013) "Rapid copper acquisition by developing murine mesothelioma: decreasing bioavailable copper slows tumor growth, normalizes vessels and promotes T cell infiltration." PLoS One. 8: e73684.
35.) Zhao XC, Dou GR, Wang L, Liang L, Tian DM, Cao XL, Qin HY, Wang CM, Zhang P, Han H. (2013) "Inhibition of Tumor Angiogenesis and Tumor Growth by the DSL Domain of Human Delta-Like 1 Targeted to Vascular Endothelial Cells."?Neoplasia. 15 :815-25
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文献和实验
31.) Guo W, Zhang D, Wang X, Kong W, Zhang Q, Zhang Y, Liu W. (2013) "Actin cytoskeleton modulates ADMA-induced NF-kappaB nuclear translocation and ICAM-1 expression in endothelial cells."?Med Sci Monit. 17: BR242
32.) Tong X, Peng H, Liu D, Ji L, Niu C, Ren J, Pan B, Hu J, Zheng L, Huang Y. (2013) "High-density lipoprotein of patients with Type 2 Diabetes Mellitus upregulates cyclooxgenase-2 expression and prostacyclin I-2 release in endothelial cells: relationship with HDL-associated sphingosine-1-phosphate."?Cardiovasc Diabetol. 12: 27.
33.) Yue X, Xu L, Zhu M, Zhang R, Liu C. (2013) "Over-expression of Nerve Growth Factor-α in human cholangiocarcinoma QBC939 cells promote tumor progression."?PLoS One. 8: e62024.
34.) Crowe A, Jackaman C, Beddoes KM, Ricciardo B, Nelson DJ. (2013) "Rapid copper acquisition by developing murine mesothelioma: decreasing bioavailable copper slows tumor growth, normalizes vessels and promotes T cell infiltration." PLoS One. 8: e73684.
35.) Zhao XC, Dou GR, Wang L, Liang L, Tian DM, Cao XL, Qin HY, Wang CM, Zhang P, Han H. (2013) "Inhibition of Tumor Angiogenesis and Tumor Growth by the DSL Domain of Human Delta-Like 1 Targeted to Vascular Endothelial Cells."?Neoplasia. 15 :815-25
为了在玻璃器内培养细胞、组织、器官或微生物和某些昆虫等,需要配制营养物质,并加入这种为培养所需的营养物质的混合物,为培养基。从生物生存发育所不可缺少的水开始,至少要求有生物体构成成分的 C、 H、 O、 N、 P、 S、 K、 Ca、 Mg、 Mn、 Fe等各种营养元素,这里除一部分可从气体中得到外,其他所有无机或有机化合物,需要从培养基中获得。需要何种化合物,要依生物的营养型,例如自养的,异养或寄生的而定。一般从营养来源因素来看,可以区分为炭源、氮源、无机盐类、发育因子等。营养物是从
HAT系次黄嘌呤( hypoxantin)、氨基蝶呤( aminopterin)和胸腺嘧啶脱氧核苷( thymidin)三种物质各英文首字之缀列, HAT培养基也就是指含有这三种物质的细胞培养基。对具有合成 DNA原料的核苷酸的形成上,在细胞内具有起始合成途径( de novo pathway)和中间合成途径( salvage pa-thway)。由于氨基蝶呤可阻碍起始合成途径,所以培养基中含有它时,细胞便只有中间合成途径,所以必须供给核苷酸。至于缺失中间合成途径的细胞,可失去增殖能力臻
培养动物细胞时,有时不用新调制的培养液,而是用已培养过多数细胞的培养液,这种培养液被认为是已由培养细胞进行了某些调整故称之为调整培养基。培养极少数细胞时,常常只有通过使用这种培养液才有可能使细胞增殖。从调整培养基中,还未单独分离出有效因子。
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