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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
上海酶研生物科技有限公司
- 英文名:
PM (Pericyte Medium)
- 规格:
500ml/T
|
货号 |
1201 |
|
产地 |
美国 |
|
缩写 |
PM |
|
规格 |
500ml |
|
用途 |
科研 |
|
储存 |
4度,-20度 |
|
运输 |
胶冰 |
周边细胞培养基是为正常人类血管周边细胞体外培养设计的适于其生长的培养基。是经灭菌的液体培养基,包含必需和非必需氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质和低浓度胎牛血清(2%)。该培养基缓冲体系为重碳酸盐,在含5%CO2的细胞培养箱中平衡后pH值为7.4。该培养基的配方能够选择性的促进正常人类周边细胞体外培养中的增殖和生长,并为其达到理想营养平衡状态提供数量上和质量上的保证。
周边细胞培养基包含500 ml基础培养基,10ml胎牛血清(FBS,目录编号0010),5ml周边细胞生长添加物(PGS,目录编号1252),和5 ml青霉素/链霉素溶液(P/S,目录编号0503)
1.) You WK, Yotsumoto F, Sakimura K, Adams RH, Stallcup WB. (2014) 'NG2 proteoglycan promotes tumor vascularization via integrin-dependent effects on pericyte function.'
2.) You WK, Yotsumoto F, Sakimura K, Adams RH, Stallcup WB. (2014) "NG2 proteoglycan promotes tumor vascularization via integrin-dependent effects on pericyte function." Angiogenesis. 17: 61-76.
3.) Tigges U, Boroujerdi A, Welser-Alves JV, Milner R. (2013) "TNF-α promotes cerebral pericyte remodeling in vitro, via a switch from 1 to 2 integrins." J Neuroinflammation. 10: 33.
4.) Mandrup OA, Friis NA, Lykkemark S, Just J, Kristensen P. (2013) "A Novel Heavy Domain Antibody Library with Functionally Optimized Complementarity Determining Regions." PLoS One. 8: e76834.
5.) Cho MK, Sun ES, Kim YH. (2013) 'Zinc-Triggered Induction of Tissue Plasminogen Activator and Plasminogen in Endothelial Cells and Pericytes.' Expneurobiol.
6.) Cho MK, Sun ES, Kim YH. (2013) "Zinc-Triggered Induction of Tissue Plasminogen Activator and Plasminogen in Endothelial Cells and Pericytes." Expneurobiol. 22: 315-21.
7.) Tigges U, Welser-Alves JV, Boroujerdi A, Milner R. (2012) 'A novel and simple method for culturing pericytes from mouse brain.'
8.) Tigges U, Welser-Alves JV, Boroujerdi A, Milner R. (2012) "A novel and simple method for culturing pericytes from mouse brain." Microvasc res. 84: 74-80.
9.) Bagley RG, Weber W, Rouleau C, Teicher BA. (2005) "Pericytes and endothelial precursor cells: cellular interactions and contributions to malignancy." Cancer Res. 65: 9741-50.
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文献和实验
1.) You WK, Yotsumoto F, Sakimura K, Adams RH, Stallcup WB. (2014) 'NG2 proteoglycan promotes tumor vascularization via integrin-dependent effects on pericyte function.'
2.) You WK, Yotsumoto F, Sakimura K, Adams RH, Stallcup WB. (2014) "NG2 proteoglycan promotes tumor vascularization via integrin-dependent effects on pericyte function." Angiogenesis. 17: 61-76.
3.) Tigges U, Boroujerdi A, Welser-Alves JV, Milner R. (2013) "TNF-α promotes cerebral pericyte remodeling in vitro, via a switch from 1 to 2 integrins." J Neuroinflammation. 10: 33.
4.) Mandrup OA, Friis NA, Lykkemark S, Just J, Kristensen P. (2013) "A Novel Heavy Domain Antibody Library with Functionally Optimized Complementarity Determining Regions." PLoS One. 8: e76834.
5.) Cho MK, Sun ES, Kim YH. (2013) 'Zinc-Triggered Induction of Tissue Plasminogen Activator and Plasminogen in Endothelial Cells and Pericytes.' Expneurobiol.
6.) Cho MK, Sun ES, Kim YH. (2013) "Zinc-Triggered Induction of Tissue Plasminogen Activator and Plasminogen in Endothelial Cells and Pericytes." Expneurobiol. 22: 315-21.
7.) Tigges U, Welser-Alves JV, Boroujerdi A, Milner R. (2012) 'A novel and simple method for culturing pericytes from mouse brain.'
8.) Tigges U, Welser-Alves JV, Boroujerdi A, Milner R. (2012) "A novel and simple method for culturing pericytes from mouse brain." Microvasc res. 84: 74-80.
9.) Bagley RG, Weber W, Rouleau C, Teicher BA. (2005) "Pericytes and endothelial precursor cells: cellular interactions and contributions to malignancy." Cancer Res. 65: 9741-50.
为了在玻璃器内培养细胞、组织、器官或微生物和某些昆虫等,需要配制营养物质,并加入这种为培养所需的营养物质的混合物,为培养基。从生物生存发育所不可缺少的水开始,至少要求有生物体构成成分的 C、 H、 O、 N、 P、 S、 K、 Ca、 Mg、 Mn、 Fe等各种营养元素,这里除一部分可从气体中得到外,其他所有无机或有机化合物,需要从培养基中获得。需要何种化合物,要依生物的营养型,例如自养的,异养或寄生的而定。一般从营养来源因素来看,可以区分为炭源、氮源、无机盐类、发育因子等。营养物是从
HAT系次黄嘌呤( hypoxantin)、氨基蝶呤( aminopterin)和胸腺嘧啶脱氧核苷( thymidin)三种物质各英文首字之缀列, HAT培养基也就是指含有这三种物质的细胞培养基。对具有合成 DNA原料的核苷酸的形成上,在细胞内具有起始合成途径( de novo pathway)和中间合成途径( salvage pa-thway)。由于氨基蝶呤可阻碍起始合成途径,所以培养基中含有它时,细胞便只有中间合成途径,所以必须供给核苷酸。至于缺失中间合成途径的细胞,可失去增殖能力臻
培养动物细胞时,有时不用新调制的培养液,而是用已培养过多数细胞的培养液,这种培养液被认为是已由培养细胞进行了某些调整故称之为调整培养基。培养极少数细胞时,常常只有通过使用这种培养液才有可能使细胞增殖。从调整培养基中,还未单独分离出有效因子。
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