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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C
- 保质期:
6个月
- 库存:
100
- 供应商:
百欧泰
- 规格:
5ml/10ml
| 规格: | 5ml | 产品价格: | ¥1500.0 |
|---|---|---|---|
| 规格: | 10ml | 产品价格: | ¥2500.0 |
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文献和实验for 5 minutes(16,000xg,4 ℃ )and discard the supernatant. 5.Wash with lysis buffer,by centrifuging 5 minutes (16,000xg,4 ℃ ),repeat wash twice. C: The crosslinking of ganglioside 1.Add 200-400 µl 50-100 uM ganglioside (diluted in PBS from 5mM in DMSO stocking
= 5’GCCTCCCTCGCGCCATCAGTATCGTAGGCACCTGAGASequence of 3’ primer= 5’AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA/iSp18/GCCTTGCCAGCCCGCTCAGTATTGATGGTGCCTACAG(purchased from Integrated DNA Technologies, Inc)0.75 uL100 uM 5’ primer0.75 uL100 uM 3’ primer10 uL10X PCR buffer (1X = 10 mM TRIS, pH8.4, 50 mM KCl, 1.5 mM
. Preparation of Adaptors a) for Taq I adaptors - make a 45 uM stock solution of adaptors - to 11 ul of 10X Gibco/BRL buffer 3 add 50 ul of the top strand adaptor (100 uM) to 50 ul of the bottom strand adaptor (100 uM). b) for Nco I adaptors
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