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- 详细信息
- 文献和实验
- 技术资料
- 规格:
50μl
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| AF5689 | Asymmetric Di-M ethyl-Histone H3 (Arg8) Rabbit Polyclonal Antibody | 50μl | 593.00元 |
| 来源 | 用途 | 交叉反应性 | 理论分子量 | 实际分子量 |
| Rabbit | WB, IF, IHC | H, M, R, Other (Wide Range) | 15kDa | 17kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
| WB | IP | IF | IHC | ICC | FC | ChIP | ChIP-seq |
| 1:500-1:2000 | - | 1:50-1:200 | 1:50-1:200 | 1:50-1:200 | - | - | - |
抗体详细信息如下::
| About this Antibody | |
| Name | Asymmetric Di-M ethyl-Histone H3 (Arg8) Rabbit Polyclonal Antibody |
| Category | Rabbit Polyclonal Antibody (pAb); Primary antibody |
| Isotype | IgG |
| Purification method | Aff.inity purification |
| Positive samples | HeLa, H3 protein |
| Cellular location | Chromosome, Nucleus |
| Customer validation | - |
| About the Immunogen | |
| Immunogen | A synthetic m ethylated peptide corresponding to residues surrounding R8 of human histone H3 |
| Sequence | - |
| Gene ID | 8290 |
| Swiss Prot | Q16695 |
| Synonyms | HIST3H3; H3.4; H3/g; H3FT; H3t; histone H3.1t |
| Category | Histone M ethylation; Crosstalk Between Protein Modifications; Epigenetics; MAPK/Erk in Growth & Differentiation |
| Background | The nucleosome is the primary chromatin building block and consists of DNA wrapped around an octamer made of paired histone proteins H2A, H2B, H3, and H4. Chromatin remodeling plays a critical role in the regulation of various nuclear activities, including transcription. Histone proteins are targets of post-translational modification, including acetylation, phosphorylation, ubiquitination, and m ethylation. Modified histone residues are recognized and bound by chromatin modifiers and the transcription machinery to regulate gene expression. Protein arginine meth yl transferases (PRMTs) met hylate histone proteins at arginine residues to generate mono-m ethylated, symmetrically di-met hylated, or asymmetrically di-meth ylated proteins. Asymmetrically di-me thylated arginine residues are found on histone H3 (Arg2, 8, 17, 26 and 42), histone H4 (Arg3), and histone H2A (Arg3) proteins. Asymmetric m ethylation is carried out by type 1 PRMTs, which include PRMT1, PRMT2, PRMT4/CARM1, and PRMT6. These modifications are often associated with actively transcribed genes. Symmetric di-me thylation of arginine residues are found on histone H3 (Arg2 and 8), Histones H4 (Arg3), and H2A (Arg3). Symmetrically di-me thylated histone arginine residues are generated by type II transferases PRMT5 and PRMT7, and are often associated with transcription repression. Arginine residues can also be deiminated by a protein arginine deiminase (PADI) to form the non-coded amino acid citrulline. Conversion of arginine to citrulline prevents m ethylation of this residue and is thought to regulate histone arginine m ethylation levels. |
包装清单:
| 产品编号 | 产品名称 | 包装 |
| AF5689 | Asymmetric Di-M ethyl-Histone H3 (Arg8) Rabbit Polyclonal Antibody | 50μl |
| AZ050 | Western一抗稀释液 | 50ml |
| — | 说明书 | 1份 |
保存条件:
Asymmetric Di-M ethyl-Histone H3 (Arg8) Rabbit Polyclonal Antibody -20ºC保存,Western一抗稀释液-20ºC或4ºC保存,一年有效。Western一抗稀释液优先推荐4ºC保存,长期不使用可以考虑-20ºC保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
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文献和实验in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
from different species and for different isotypes within a species. Tables I may be used to determine relative avidity for polyclonal antibodies of a given species. Table II shows relative affinities for varius isotypes.
Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
to chromatin, by its histone tail interaction or by other interactions, then you would not expect to see loss of binding upon peptide competition. A good positive control for the assay is to use H3K9 dimethylated peptide to compete HP1 from the pericentric











