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Asymmetric Di-M ethyl-Histone

H3 (Arg8) Rabbit Polyclonal Antibody
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  • ¥593
  • 碧云天(beyotime)已认证
  • AF5689
  • 2026年01月07日
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      50μl

    产品编号 产品名称 产品包装 产品价格
    AF5689 Asymmetric Di-M ethyl-Histone H3 (Arg8) Rabbit Polyclonal Antibody 50μl 593.00元
    来源 用途 交叉反应性 理论分子量 实际分子量
    Rabbit WB, IF, IHC H, M, R, Other (Wide Range) 15kDa 17kDa

    WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing.
    H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
    配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
    建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):

    WB IP IF IHC ICC FC ChIP ChIP-seq
    1:500-1:2000 - 1:50-1:200 1:50-1:200 1:50-1:200 - - -

    抗体详细信息如下::

    About this Antibody
    Name Asymmetric Di-M ethyl-Histone H3 (Arg8) Rabbit Polyclonal Antibody
    Category Rabbit Polyclonal Antibody (pAb); Primary antibody
    Isotype IgG
    Purification method Aff.inity purification
    Positive samples HeLa, H3 protein
    Cellular location Chromosome, Nucleus
    Customer validation -
    About the Immunogen
    Immunogen A synthetic m ethylated peptide corresponding to residues surrounding R8 of human histone H3
    Sequence -
    Gene ID 8290
    Swiss Prot Q16695
    Synonyms HIST3H3; H3.4; H3/g; H3FT; H3t; histone H3.1t
    Category Histone M ethylation; Crosstalk Between Protein Modifications; Epigenetics; MAPK/Erk in Growth & Differentiation
    Background The nucleosome is the primary chromatin building block and consists of DNA wrapped around an octamer made of paired histone proteins H2A, H2B, H3, and H4. Chromatin remodeling plays a critical role in the regulation of various nuclear activities, including transcription. Histone proteins are targets of post-translational modification, including acetylation, phosphorylation, ubiquitination, and m ethylation. Modified histone residues are recognized and bound by chromatin modifiers and the transcription machinery to regulate gene expression. Protein arginine meth yl transferases (PRMTs) met hylate histone proteins at arginine residues to generate mono-m ethylated, symmetrically di-met hylated, or asymmetrically di-meth ylated proteins. Asymmetrically di-me thylated arginine residues are found on histone H3 (Arg2, 8, 17, 26 and 42), histone H4 (Arg3), and histone H2A (Arg3) proteins. Asymmetric m ethylation is carried out by type 1 PRMTs, which include PRMT1, PRMT2, PRMT4/CARM1, and PRMT6. These modifications are often associated with actively transcribed genes. Symmetric di-me thylation of arginine residues are found on histone H3 (Arg2 and 8), Histones H4 (Arg3), and H2A (Arg3). Symmetrically di-me thylated histone arginine residues are generated by type II transferases PRMT5 and PRMT7, and are often associated with transcription repression. Arginine residues can also be deiminated by a protein arginine deiminase (PADI) to form the non-coded amino acid citrulline. Conversion of arginine to citrulline prevents m ethylation of this residue and is thought to regulate histone arginine m ethylation levels.

    包装清单:

    产品编号 产品名称 包装
    AF5689 Asymmetric Di-M ethyl-Histone H3 (Arg8) Rabbit Polyclonal Antibody 50μl
    AZ050 Western一抗稀释液 50ml
    说明书 1份

    保存条件:
    Asymmetric Di-M ethyl-Histone H3 (Arg8) Rabbit Polyclonal Antibody -20ºC保存,Western一抗稀释液-20ºC或4ºC保存,一年有效。Western一抗稀释液优先推荐4ºC保存,长期不使用可以考虑-20ºC保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
    注意事项:
    如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
    回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
    提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果,请考虑使用上述检测专用的一抗稀释液。
    本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
    为了您的安全和健康,请穿实验服并戴一次性手套操作。

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