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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20℃,2 years.(Avoid freeze/thaw cycles)
- 保质期:
Store at -20℃,2 years.(Avoid freeze/thaw cycles)
- 英文名:
FFAGLDD
- 库存:
现询
- 供应商:
北京索莱宝科技有限公司
- 规格:
10mg/5mg/1mg
| 规格: | 10mg | 产品价格: | ¥3100.0 |
|---|---|---|---|
| 规格: | 5mg | 产品价格: | ¥2220.0 |
| 规格: | 1mg | 产品价格: | ¥890.0 |
| 基本信息 | |
| 产品类型 | 多肽 |
| 单字母序列 | AKSKWKQAFNATAVVRHMRKLQ |
| 三字母序列 | Ala-Lys-Ser-Lys-Trp-Lys-Gln-Ala-Phe-Asn-Ala-Thr-Ala-Val-Val-Arg-His-Met-Arg-Lys-Leu-Gln |
| 分子式 | C116H192N38O28S1 |
| 分子量 | 2599.1 |
| 纯度 | ≥95% |
| 外观(性状) | 冻干粉;Lyophilized powder |
| 盐体系 | TFA盐;Trifluoroacetate salt |
| 来源 | 合成;Synthetic |
| 储存条件 | Store at -20℃,2 years.(Avoid freeze/thaw cycles) |
| 类别/标签 | 激素和相关肽(Hormone and Related Peptides) |
| 规格 | This is amino acids 299 to 320 fragment of the calmodulin-dependent protein kinase I (CaM kinase I) binding domain. CaM kinase I is a member of the expanding class of protein kinases that is regulated by calmodulin (CaM). This peptide is a putative CaM-binding region. |
| In Vitro | 未检测 |
| 单位 | 瓶 |
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文献和实验Use of Peptide Libraries to Determine Optimal Substrates of Tyrosine Kinases
of 110 KDa; this interaction leads to phosphorylation of the protein by the catalytic domain of Src (4 ). Similarly, the SH2 domain of the Abl tyrosine kinase is required for hyperphosphorylation of rts substrate p130CAS (5 ). Substitutions of SH
medium (I, e.g., Dulbecco's modified Eagle medium) 11. FLAG peptide (5 mg/ml in hypotonic lysis buffer) (I) 12. Kinase buffer containing benzamidine, 10 mm and 100 mm NaCl (II) 13. 2x Laemmli sample buffer (I) 14. Hypotonic lysis
同位素法测定底物磷酸化活性方法 Phosphorylation of Substrates
(I) and ice cold (I+II) (see Protocol 1) Serum-free medium (I, e.g., Dulbecco's modified Eagle medium) FLAG peptide (5 mg/ml in hypotonic lysis buffer) (I) Kinase buffer containing benzamidine, 10 mm and 100 mm NaCl
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