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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
详询
- 库存:
50
- 供应商:
广州市左克生物
- 规格:
25次
产品简介:
品牌:ZYMO RESEARCH/简石
货号:S6011
产品名称:ZR BashingBead™ Lysis/Filtration Tubes w/ 2.0 mm Beads (50 ml) (25 Pack)
品牌介绍:
ZYMO RESEARCH生物公司位于美国加州Orange County,创建于上世纪797年代初,主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒。 其DNA甲基化,羟甲基化,核酸提取等产品已得到世界各实验室的认可,产品以“快速、简便、超纯”的特点在众多生物公司中脱颖而出,独占鳌头。
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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
Inc.) or QiaFilter™ (Qiagen Inc.) plate positioned over a 96 deep-well collection block inside a vacuum manifold. Transfer 600 ul at a time, collecting the top half (with flocculent ppt.) first. Vacuum filter using approximately 300-500 mm Hg until filtration
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
.) first. Vacuum filter using approximately 300-500 mm Hg until filtration is complete, about 5-20 minutes. 8. Remove lysate filtrate collection block from manifold. Add 2ul of glycogen type II (Sigma Inc.) at 1ug/ul and ice-cold isopropanol (0.7x
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmids*
. Alternatively, 24 deep-well or 48 deep-well blocks may be used with up to 4.8 or 2.4ml of media respectively. Cover each block with an AirPore™ (Qiagen Inc.) sheet and incubate at 37�C with shaking at 325 RPM for approximately 20 hours. 2. Pellet cells
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