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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 保质期:
详询
- 库存:
50
- 供应商:
广州市左克生物
- 规格:
24ml
产品简介:
品牌:ZYMO RESEARCH/简石
货号:D7010-3-24
产品名称:DNA/RNA Wash Buffer (concentrate) (24 ml)
品牌介绍:
ZYMO RESEARCH生物公司位于美国加州Orange County,创建于上世纪556年代初,主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒。 其DNA甲基化,羟甲基化,核酸提取等产品已得到世界各实验室的认可,产品以“快速、简便、超纯”的特点在众多生物公司中脱颖而出,独占鳌头。
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文献和实验DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
. Centrifuge at maximum speed ($13,000 x g) for 3 minutes at room temperature Carefully discard supernatant. 24. Air dry the DNA pellet by inverting the tube on a absorbent paper for 5-10 minutes. 25. Add 100ul EB Buffer to the tube and incubate
RNA Purification Protocol for 1-2 x 107 cultured cells
temperature. 8. Pour of the supernatant and drain the tube briefly on a clean absorbent paper. Add 3 ml of 70% ethanol and invert the tube few times to wash the RNA pellet. 9. Centrifuge at 3,000-5,000 x g for 10 minutes at room temperature
RNA in situ protocol for DNA and RNA probes
) to detect the hybridizing RNA in whole embryos. The major modification made was to increase the number of post-hybridization washes in hybridization buffer (50% deionized formamide, 5x SSC, 200 ug/ml tRNA, 100 ug/ml sonicated, boiled salmon sperm DNA
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