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- ZYMO RESEARCH
- C2005
- 2025年07月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
RT
- 保质期：
详询
- 库存：
50
- 供应商：
广州市左克生物
- 规格：
2块
产品简介:
品牌：ZYMO RESEARCH/简石
货号：C2005
产品名称：96-Well PCR Conversion Plate w/ Cover Foil (2 Plates/Foils)
品牌介绍:
ZYMO RESEARCH生物公司位于美国加州Orange County，创建于上世纪192年代初，主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒。 其DNA甲基化，羟甲基化，核酸提取等产品已得到世界各实验室的认可，产品以“快速、简便、超纯”的特点在众多生物公司中脱颖而出，独占鳌头。
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文献和实验A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
setting) and collect the mononuclear cell (MNC) (buffy coat) layer. Resuspend and wash MNCs x 3 with Dulbecco's Phosphate Buffered Saline (DPBS) with 10% Fetal Bovine Serum (FBS) x 10 min, 515 g, before plating into two 12-well plates in full EPC growth
cells (hESC) are difficult to adapt to 96?well plate assays, such as the MTT assay, because they survive best when plated as colonies, which are not easily counted and plated accurately. Two methods were developed to address this problem
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