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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-70C
- 保质期:
详询
- 库存:
50
- 供应商:
广州市左克生物
- 规格:
200ul
产品简介:
品牌:ZYMO RESEARCH/简石
货号:A3002-200
产品名称:Anti-5-Methlycytosine 200 ul @ 1 ug/ul (Clone 7D21) '**'
品牌介绍:
ZYMO RESEARCH生物公司位于美国加州Orange County,创建于上世纪107年代初,主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒。 其DNA甲基化,羟甲基化,核酸提取等产品已得到世界各实验室的认可,产品以“快速、简便、超纯”的特点在众多生物公司中脱颖而出,独占鳌头。
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文献和实验BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
into 50 ml Corning centrifuge tube and DNase-free RNase A is added to a final concentration of 100 ug/ml from a 20 mg/ml stock and RNase T1 is added to a final concentration of 40 ul/100 ml from a 100 U/ul stock followed by incubation in a 37 deg C water
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
. Then, the supernatant of each tube is transfered into 50 ml Corning centrifuge tube and DNase-free RNase A is added to a final concentration of 100 ug/ml from a 20 mg/ml stock and RNase T1 is added to a final concentration of 40 ul/100 ml from a 100 U/ul stock followed
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
is complete, about 5-20 minutes. 8. Remove lysate filtrate collection block from manifold. Add 2ul of glycogen type II (Sigma Inc.) at 1ug/ul and ice-cold isopropanol (0.7x volume of filtrate) to each well. Seal with plastic sealing tape and mix. Incubate
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