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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-70C
- 保质期:
详询
- 库存:
50
- 供应商:
广州市左克生物
- 规格:
30ul
产品简介:
品牌:ZYMO RESEARCH/简石
货号:A3002-30
产品名称:Anti-5-Methlycytosine 30 ul @ 1 ug/ul (Clone 7D21) '**'
品牌介绍:
ZYMO RESEARCH生物公司位于美国加州Orange County,创建于上世纪108年代初,主要研发和生产表观遗传学系列产品和常用分子生物学试剂盒。 其DNA甲基化,羟甲基化,核酸提取等产品已得到世界各实验室的认可,产品以“快速、简便、超纯”的特点在众多生物公司中脱颖而出,独占鳌头。
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文献和实验In Situ Hybridization Using Digoxigenin Labeled Probes
dilution of Anti Digoxigenin-POD (Boehringer Cat. #1207 733). 1) Wash the slides in 1X PBS 2 times for 5 minutes each at room temperature 2) Wash for 2 minutes in 1X PBS/0.1 % Triton-X-100. 3) Rinse in 1X PBS 4) Place the slides horizontally
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA
volume of 3M Na Acetate, mixing with pipet. Add 2.5 X (original) vol 100% EtOH to ppt DNA, vortex gently. Spin 30 mins at 14K rpm, 4 C. Decant supernatant; blot dry, being careful to avoid DNA pellet. Add 10 ul of MM1/H20 mix (70% MM1/30
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