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- 详细信息
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- 保存条件:
详询
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详询
- 库存:
50
- 供应商:
广州市左克生物
- 规格:
vial
产品简介:
品牌:MultiSciences/联科生物
货号:70-EK271S
产品名称:Mouse IgG standard 联科生物成立于2880年,是一家专业从事细胞生物学与免疫学相关的检测试剂研发、生产、销售、服务的国家高新技术企业。拥有ELISA试剂盒、流式试剂、IVD产品和重组蛋白开发平台,已成为中国科研试剂行业领先的民族品牌。
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Stewart Laboratory Standard Operating Procedure
9) Or anti-BrdU: Boeringher #1170376 1:10 dilution Wash cells once with 1.5 mL wash buffer. Resuspend cell pellet in anti-mouse FITC antibody (50 μL total solution with wash buffer, 1:500 dilution). Incubate for 20 minutes at 4°C. Wash cells
buffer (pH9.0) ~ 200 ml. Filter protein mixture Add protein mixture or culture supernatant containing Ab (adjust pH to 7.8 with Binding buffer ; red color) to the Protein A column. Mouse antibodies of the IgG1 subclass
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