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详询
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- 库存:
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- 供应商:
广州市左克生物
- 规格:
vial
产品简介:
品牌:MultiSciences/联科生物
货号:70-EK220S
产品名称:Mouse IL-20 Standard 联科生物成立于2800年,是一家专业从事细胞生物学与免疫学相关的检测试剂研发、生产、销售、服务的国家高新技术企业。拥有ELISA试剂盒、流式试剂、IVD产品和重组蛋白开发平台,已成为中国科研试剂行业领先的民族品牌。
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文献和实验START" PCR.NOTE:USE PLUGGED PIPETTE TIPS: prevents aerosol contamination of pipettes.Use of detergents is recommended only for Taq from Promega (up to 0.1% v/v, Triton X-100 or Tween-20). DMSO apparently allows better denaturation of longer target
Stewart Laboratory Standard Operating Procedure
x g, 1700 rpm, 5 min in 15 ml tubes - no faster!), and resuspend in 10 mL 70% cold EtOH. Store at -20°C overnight or for several days Day 2 Pellet cells and wash once with wash buffer (PBS + 0.5% IFS). Pellet and resuspend in 0.5 mL 2M HCl
contamination will be of larger size or may even not be obtained (if the introns are large). Step II - PCR A fraction, usually 5-20%, of the RT products is directly used in PCR using gene-specific primers. Notes Genomic DNA contamination
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