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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human IL-35 (IL12A & EBI3 Heterodimer) Protein
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
50.00 µg///500.00 µg
蛋白名称:Human IL-35 (IL12A & EBI3 Heterodimer) Protein, Endotoxin-Free
蛋白构建:A DNA sequence encoding the p35 subunit of human IL12, termed as IL12A (P29459-1) (Met1-Ser219) was fused with a C-terminal Fc region of human IgG1 tag followed by a His tag, constructed the plasmid 1; A DNA sequence encoding the human IL27B, (NP_005746.2) (Met1-Lys229) was fused with a C-terminal Fc region of human IgG1 tag followed by a FLAG tag, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
表达宿主:HEK293 Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE.
蛋白内毒素:< 0.5 EU per mg protein.
预测N端:Arg 23 & Arg 21
蛋白分子量:The recombinant human IL12A&IL27B comprises 905 (447+458) amino acids and has a calculated molecular mass of 102 (50.7+ 51.3) KDa.
蛋白NP号:P29459-1 & NP_005746.2
蛋白氨基酸序列:Met1-Ser219 & Met1-Lys229
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Metabolic Labeling of Cells with 35S
1) Transfer to a 24 wells plate the desired colonies. 2) Once the cells are attached (at least 8 hours after tripsinizing them) add ~1 ml of DME (met-, cys-). 3) Add 3l of Met 35S, Cys 35S trans label. 4) Incubate at least 5 hours at 370C
x2 检验 一、目的要求 1.明确x2 检验的意义和用途。 2.掌握x2 检验方法。 二、内容 [选择题] 1.四格表中四个格子基本数字是。 (1)两个样本率的分子和分母; (2)两个构成比的分子和分母; (3)两对实测阳性绝对数和阴性绝对数;(4)两对实测数和理论数。 2.四个百分率作比较,有1个理论数小于5,大于1,其它都大于
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