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- Solarbio
- P00287
- 北京
- 2025年07月25日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 英文名：
Recombinant Human Annexin V
- 规格：
100ug
| 基本信息 | |
| 基因名 | |
| 英文名称 | Recombinant Human Annexin V |
| 纯度 | ＞90% as determined by reducing SDS-PAGE. |
| 储存条件 | Reconstituted protein solution should be stored at < -20°C for 1year, though stable at 2-8°C for 1 month. |
| 运输条件 | The product is shipped at 2-8°C temperature. Upon receipt, store it immediately at the temperature listed below. |
| 浓度 | 1 mg/mL as determined by BCA. |
| 来源 | E.coli |
| 单位 | 支 |
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文献和实验Purification of Annexin V and Its Use in the Detection of Apoptotic Cells
, but not necrosis, allowing differentiation between the two types of cell death. In this chapter we detail two methods for the purification of annexin V. The first is an untagged recombinant protein using a three step Fast Protein Liquid Chromatography (FPLC) method
的核酸染料(标记的Annexin V与核酸染料均需要摸索到合适的使用浓度,商品化试剂盒按照说明书操作); 4、均匀混合,室温避光反应15分钟;(若是用Annexin V-Biotin标记蛋白染色,反应后用1X的结合缓冲液洗涤细胞一次,再加入合适浓度的标记链霉亲和素Streptavidin,再加入合适的核酸染料,室温避光反应15分钟) 5、向流式管中加入400μl 1X的结合缓冲液,重悬后及时上流式细胞仪进行分析. 建议设置
Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I)
The development of efficient recombinant protein production processes can be a critical factor in whether or not a pharmaceutical therapeutic protein can enter human clinical trials and ultimately the marketplace. This is especially true
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