- ￥468 - 4160
- Solarbio
- P00414
- 北京
- 2025年07月25日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 规格：
1mg/500ug/50ug/10ug
| 规格： | 1mg | 产品价格： | ￥4160.0 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ￥2600.0 |
| 规格： | 50ug | 产品价格： | ￥936.0 |
| 规格： | 10ug | 产品价格： | ￥468.0 |
| 基本信息 | |
| 基因名 | |
| 别名 | Myeloperoxidase;MPO |
| 中文名称 | Recombinant Human Myeloperoxidase/MPO |
| 外观(性状) | Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH8.0. |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 运输条件 | The product is shipped at 2-8°C temperature. Upon receipt, store it immediately at the temperature listed below. |
| 背景说明 | Myeloperoxidase (MPO) is a heme-containingenzyme belonging to the XPO subfamily of peroxidases.It is an abundant neutrophil and monocyte glycoprotein thatcatalyzes the hydrogen peroxide-dependentconversion of chloride，bromide，and iodide to multiple reactive species.Post-translationalprocessing of MPO involvesthe insertion of a heme moiety and the proteolytic removal of both a propeptide and a 6 aa internal peptide.This results in a disulfide-linkeddimer composed of a60 kDa heavy and 12 kDa light chain that associate into a 150 kDa enzymatically active tetramer.The tetramer contains two heme groups and one disulfide bondbetween the heavy chains.Alternate splicing generates two additional isoforms of MPO，one with a 32 aa insertion in the light chain，and another with a deletion ofthe signal sequence and part of the propeptide.Human and mouse MPO share 87% aa sequence identity.MPO activity results in protein nitrosylation and theformation of 3-chlorotyrosineand dityrosine crosslinks.MPO is also associated with a variety of other diseases，and inhibits vasodilation in inflammation by depleting the levelsof NO.Serum albumin functions as a carrier protein during MPO movement to the basolateral side of epithelial cells.MPO is stored in neutrophil azurophilicgranules.Upon cellular activation，it is deposited into pathogen-containingphagosomes. |
| 来源 | Human Cells |
| 溶剂 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 内毒素 | Less than 0.1 ng/ug(1 EU/ug)as determined by LAL test. |
| 单位 | 支 |
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我们知道，PeproTech的所有细胞因子和蛋白均为冻干粉，这使得运输非常便捷，只要常温即可。而且，细胞因子和蛋白冻干粉非常稳定，在-20℃或-80℃条件下可保存数年。冻干粉在使用前需进行溶解，然后以液体形式加到培养体系或注射入动物体内。溶解步骤非常关键，因溶解不好会导致细胞因子或蛋白的失活，这也是很多用户在实际使用中经常遇到的问题。 那么，应该如何进行正确的溶解呢？ 下面我们以Recombinant Human IL-4 (重组人IL-4，产品编号：200-04)的说明书为例
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