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- Solarbio
- P00067
- 北京
- 2025年07月25日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- 规格：
1mg/500ug/50ug/10ug
| 规格： | 1mg | 产品价格： | ￥21120.0 |
|---|---|---|---|
| 规格： | 500ug | 产品价格： | ￥14784.0 |
| 规格： | 50ug | 产品价格： | ￥4576.0 |
| 规格： | 10ug | 产品价格： | ￥1560.0 |
Recombinant Human Oncostatin M is produced by our E.coli expression system and the target gene encoding Ala26-Arg221 is expressed with a 6His tag at the N-terminus.
| 基本信息 | |
| 基因名 | |
| 别名 | Oncostatin-M;OSM |
| 外观(性状) | Lyophilized from a 0.2 μm filtered solution of 20mMTris-HCl, 1mM EDTA, 200mM NaCl, pH 7.5. |
| 纯度 | ≥95% as determined by reducing SDS-PAGE. |
| 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 运输条件 | The product is shipped at 2-8°C temperature. Upon receipt, store it immediately at the temperature listed below. |
| 背景说明 | Oncostatin M (OSM) is a glycoprotein belonging to the interleukin-6 family of cytokines that includes leukemia-inhibitory factor，granulocyte colony-stimulating factor，and interleukin 6.OSM encodes a growth regulator，which Inhibits the proliferation of a number of tumor cell lines.It stimulates proliferation of AIDS-KS cells.OSM regulates cytokine production，including IL-6，G-CSF and GM-CSF from endothelial cells.OSM is considered as a pleiotropic cytokine that initiates its biological activities through specific cell surface receptors.The low affinity LIF receptor that shares the similarity of containing protein gp130 has now been identified to be a component of a high- affinity OSM receptor that will transduce OSM signals.OSM has also been shown to play a role in both pro and anti-inflammatory actions.OSM may also be involved in many biometabolism processes including liver development，haematopoeisis，inflammation，bone formation and destruction and possibly CNS development. |
| 来源 | E.coli |
| 溶剂 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 μg/ml Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 内毒素 | Less than 0.1 ng/ug(1 EU/ug)as determined by LAL test. |
| 单位 | 支 |
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文献和实验水平。根据试剂盒说明书，用生长于或未生长于MEFs上的未分化ES细胞制备细胞核提取物。用Bradford检测对蛋白质进行定量分析。STAT3活化水平根据产品说明书而确定，然后根据总蛋白水平进行标准化处理。 小鼠嵌合体的生产：所有程序由密西根大学动物使用和照顾委员会批准。根据国家研究委员会关于实验动物照顾和使用指南概述的原则和程序对动物提供照顾。在含有LIF或者OSM的培养基中连续传代5次后，用胰蛋白酶处理细胞，然后将16个ES细胞显微注射到囊胚中。囊胚取自与C57
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