IT3780 硫黄素T 染料/探针 索莱宝

当Thioflavin T与β片层结合时，例如淀粉样蛋白寡聚体中的那些，染料经历其激发光谱的特征性的120nm红移，其可以在450nm处被选择性激发，产生482nm的荧光信号。Thioflavin T快速且特异性结合由合成β-淀粉样蛋白（1-40）形成的反平行β-折叠原纤维，但不结合单体或寡聚中间体。Thioflavin T的结合不会干扰β-淀粉样肽聚集成淀粉样蛋白原纤维。该染料常用于可视化由阿尔茨海默病患者脑中发现的β-淀粉样蛋白组成的斑块。

使用本产品的案例（仅供参考）

In Vitro

Aβ25–35 aggregation assay（20 μM Aβ25–35；20 μM Th-T solution）

Th-T is a relatively common fluorescent dye for detecting Aβ aggregation.   The experiment was divided into three parts.   

①. Aβ25–35 aggregation assay: 20 μM of Aβ25–35 with and without baicalein (80 μM) were incubated at 37 °C for 6 days.   After incubation, 10 μl of the mixture was added into 190 μl of Th-T solution (20 μM) in a black 96-well plate.   The fluorescence intensity was measured daily at the same time for 6 consecutive days.   

②. Anti-aggregation assay of baicalein: A mixture of Aβ25–35 (20 μM) and baicalein (40, 60 and 80μM) was prepared in PBS, then the mixture was incubated at 37 °C for 6 days.   10 μl of the mixture was added into 190 μl of Th-T solution (20 μM), and the fluorescence intensity of the mixture was then measured.   

③. Disaggregation of Aβ25–35 amyloid fibrils by baicalein: Aβ25–35 (20 μM) solution was incubated alone for 3 days, then it was incubated with baicalein (40, 60 and 80 μM) for another 2 days at 37 °C.   After incubation 3 days or 5 days, 10 μl of the mixture was added into 190 μl of Th-T solution (20 μM), and the fluorescence intensity was measured using a microplate reader at 440 nm excitation and 490 nm emission.

来源文献：Gao L, Zhou F, Wang KX, Zhou YZ, Du GH, Qin XM. Baicalein protects PC12 cells from Aβ25-35-induced cytotoxicity via inhibition of apoptosis and metabolic disorders. Life Sci. 2020 May 1;248:117471. doi: 10.1016/j.lfs.2020.117471. Epub 2020 Feb 26. PMID: 32112868.

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标题:Structural analysis and cellular visualization of APP RNA G-quadruplex

成员:Lyu K; Chen SB; Chan CY; Tan JH; Kwok CK

论文因子:9.452 发表期刊:Chem Sci pmid:32206258

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标题:Polarity-activated super-resolution imaging probe for the formation and morphology of amyloid fibrils[J]

成员:Lv Z ; Li L ; Man Z ; et al

论文因子:8.231 发表期刊:Nano research

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标题:Porous carbon nanotube microspheres with tailorable surface wettability areas for oil adsorption

成员:Ye X; Shao C; Fan Q; Shang L; Ye F

论文因子:7.872 发表期刊:J Colloid Interface Sci pmid:34293531

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标题:Ligustilide alleviates neurotoxicity in SH-SY5Y cells induced by Aβ25-35 via regulating endoplasmic reticulum stress and autophagy

成员:Gao LJ; Li P; Ma T; Zhong ZQ; Xu SJ

论文因子:5.502 发表期刊:Phytother Res pmid:33111362

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标题:Baicalein protects PC12 cells from Aβ25-35-induced cytotoxicity via inhibition of apoptosis and metabolic disorders

成员:Gao L; Zhou F; Wang KX; Zhou YZ; Du GH; Qin XM

论文因子:4.659 发表期刊:Life Sci pmid:32112868

基本信息
CAS	No.2390-54-7
中文名称	硫黄素T
英文名称	Thioflavin T
别名	硫黄素T;碱性黄1;硫代磺素T;硫代黄色素T;盐基嫩黄;
分子式	C17H19ClN2S
分子量	318.86
溶解性	Soluble in Water/DMSO(Need ultrasonic)
纯度	≥98%
外观(性状)	Solid
储存条件	Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
MDL	MFCD00011944
SMILES	CC1=CC2=C(C=C1)[N+](=C(S2)C3=CC=C(C=C3)N(C)C)C.[Cl-]
InChIKey	JADVWWSKYZXRGX-UHFFFAOYSA-M
InChI	InChI=1S/C17H19N2S.ClH/c1-12-5-10-15-16(11-12)20-17(19(15)4)13-6-8-14(9-7-13)18(2)3;/h5-11H，1-4H3;1H/q+1;/p-1
PubChem CID	16953
背景说明	是一种阳离子 Benzothiazole 染料，在组织切片中与淀粉样蛋白结合后荧光强度增强，通常用于诊断淀粉样蛋白原纤维的相关研究。。
单位	瓶