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- 技术资料
- 保存条件：
Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
- 保质期：
Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year
- 英文名：
H-89
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- CAS号：
127243-85-0
- 规格：
10mg
| 基本信息 | |
| CAS | No.127243-85-0 |
| 英文名称 | H-89 |
| 分子式 | C20H20BrN3O2S |
| 分子量 | 446.36 |
| 溶解性 | Soluble in DMSO |
| 纯度 | ≥98% |
| 外观(性状) | Solid |
| 储存条件 | Powder:2-8℃，2 years;Insolvent(母液):-20℃，6 months;-80℃，1 year |
| MDL | MFCD00214120 |
| SMILES | C1=CC2=C(C=CN=C2)C(=C1)S(=O)(=O)NCCNC/C=C/C3=CC=C(C=C3)Br |
| InChIKey | ZKZXNDJNWUTGDK-NSCUHMNNSA-N |
| InChI | InChI=1S/C20H20BrN3O2S/c21-18-8-6-16(7-9-18)3-2-11-22-13-14-24-27(25，26)20-5-1-4-17-15-23-12-10-19(17)20/h1-10，12，15，22，24H，11，13-14H2/b3-2+ |
| PubChem CID | 449241 |
| 靶点 | PKA |
| 通路 | Stem Cells; Protein Tyrosine Kinase/RTK |
| 背景说明 | H-89 是一种有效、选择性的 cAMP 依赖性 protein kinase A 抑制剂。 |
| 生物活性 | H-89 is a potent and selective inhibitor of cyclic AMP-dependent protein kinase (protein kinase A) with IC50 of 48 nM and has weak inhibition on PKG， PKC， Casein Kinase， and others kinases.[1-3] |
| IC50 | IC50: 48 nM (protein kinase A)[1-3] |
| In Vitro | H-89 inhibits protein kinase A， in competitive fashion against ATP. H-89 causes a dose-dependent inhibition of the forskolin-induced protein phosphorylation， with no decrease in intracellular cyclic AMP levels in PC12D cells. H-89 significantly inhibits the forskolin-induced neurite outgrowth from PC12D cells. H-89 (30 μM) inhibits significantly cAMP-dependent histone IIb phosphorylation activity in PC12D cell lysates[1]. H-89 (1-2 μM) significantly slows the repriming rate in rat skinned fibres， most likely due to it deleteriously affecting the T-system potential. H-89 (10-100 μM) inhibits net Ca2+ uptake by the SR and affectes the Ca32-sensitivity of the contractile apparatus in rat skinned fibres[2]. |
| 细胞实验 | H-89 (0.2 mg/100g， i.p.) significantly increases seizure latency and threshold in PTZ-treated animals. H-89 (0.05， 0.2 mg/100 g， i.p.) prevents the epileptogenic activity of bucladesine (300 nM) with significant increase of seizure latency and seizure threshold[3]. |
| 细胞实验 | After 48 h in culture， PCl2D cells are cultured in test medium containing 30 μM H-89 for 1 h and then exposed to fresh medium that contained both 10 μM forskolin and 30 μM H-89. Cells are scraped off with a rubber policeman and sonicated in the presence of 0.5 mL of 6% trichloroacetic acid. To extract trichloroacetic acid， 2 mL of petroleum ether is added， the preparation mixed and centrifuged at 3000 rpm for 10 min. After aspiration of the upper layer， the residue sample solution is used for determination.[1-3] |
| 动物实验 | Male albino mice weighing 20-25 g are obtained. Pentoxifylline (25， 50， 100 mg/kg)， bucladesine (50， 100， 300 nM/mouse) and H-89 (0.05， 0.1， 0.2 mg/100 g) are administered intraperitoneally (i.p.) 30 min before intravenous (i.v.) infusion of PTZ. In combination groups， the first and second components are injected 45 and 30 min before PTZ infusion. In all groups， the respective control animalsreceive an appropriate volume of vehicle. For the i.v. infusion， the needle is inserted into the lateral tail vein， fixed to the tail vein by a narrow piece of adhesive tape， and the animal is allowed to move freely. PTZ solution is infused at a concentration rate of 1 mL/min.[1-3] |
| 激酶实验 | Kinase activities are assayed at 30°C for 2-5 min by measuring the transfer of 32P from [γ-32P]ATP to substrates. The reaction is terminated by adding 1 mL of 20% trichloroacetic acid， following the addition of 100 μg of bovine serum albumin as a carrier protein. The sample is centrifuged at 3000 rpm for 10 min， the pellet is resuspended in 5% trichloroacetic acid solution， the final pellet is dissolved in 1 mL of 1 N NaOH and the radioactivity is measured in a liquid scintillation counter.[1-3] |
| 数据来源文献 | [1]. Chijiwa T， et al. Inhibition of forskolin-induced neurite outgrowth and protein phosphorylation by a newly synthesized selective inhibitor of cyclic AMP-dependent protein kinase， N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H-89)， of PC12D [2]. Blazev R， et al. Effects of the PKA inhibitor H-89 on excitation-contraction coupling in skinned and intact skeletal muscle fibres. J Muscle Res Cell Motil. 2001;22(3):277-86. [3]. Hosseini-Zare MS， et al. Effects of pentoxifylline and H-89 on epileptogenic activity of bucladesine in pentylenetetrazol-treated mice. Eur J Pharmacol. 2011 Nov 30;670(2-3):464-70. |
| 单位 | 瓶 |
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IH1730 H-89 抑制剂/拮抗剂/激动剂 索莱宝
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