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Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus (SI M-MLV)是从克隆有经基因改造的Moloney Murine Leukemia Virus reverse transcriptase基因载体在大肠杆菌中经诱导表达分离纯化而来。该酶可在37-42℃用于cDNA第一链的合成,提供持续的特异性的扩增,高效的生产cDNA,它可反转录比其它酶更长的片段,cDNA片段的长度在100bp~12kb范围之间。其最佳反应温度是37℃,具有灵敏度高、特异性强、聚合活性高和半衰期长的特点
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文献和实验M-MLV第一链cDNA合成试剂盒-技术手册一、 试剂盒组成 Components SK2027 SK2028 5×M-MLV Reaction Buffer 100µl
SI Mapping Using Single-Stranded DNA Probes
The SI nuclease is an endonuclease isolated from Aspergillus oryzae that digests single‐ but not doublesdeshstranded nucleic acid. In addition, it digests partially mismatched double‐stranded molecules with such sensitivity that even a single
approaches used for capillary coating and concentrates on those utilizing Si-O and Si-C covalent bonds. The apparatus and methods are presented for capillary coating using Si-O and Si-C chemistry. Furthermore, procedures are described for monitoring
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