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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃
- 供应商:
北京博蕾德生物科技有限公司
- 抗体英文名:
IgEQUANT-R(Receptor based Human IgE ELISA Kit)
- 抗体名:
IgEQUANT-R(Receptor based Human IgE ELISA Kit)
- 规格:
1Kit
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文献和实验This protocol is based on enrichment from 1 x 107 leucocytes (MNC). It is scalable from 1 x 107 –2 x 109 cells. Keep the cells and buffers cold (2–8°C) during the whole process. 1) Transfer 100 μl (1 x 107 ) leucocytes in Buffer
ELISA-based assay for scatchard analysis of ligand-receptor interactions
A simple, nonradioactive method is presented that can be used for performing large numbers of binding assays of cell membrane receptors with their ligands. The method adopts the simple membrane preparation and biotin-based quantitation methods
反应发生的场所。IgE为亲细胞抗体,Cε2和Cε3功能区可与嗜碱性粒细胞、肥大细胞膜上高亲和力FcεRⅠ结合。变应原再次进入机体与已固定在嗜碱性粒细胞、肥大细胞上IgE结合,可引起Ⅰ型变态反应。寄生虫感染或过敏反应发作时,局部的外分泌液和血清中IgE水平都明显升高。
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