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文献和实验Mag-Bind® mRNA Protocol (Standard Protocol)
capable of 12,000 x g and 2-8°C 实验步骤 Total RNA Isolation: 1. Homogenization and lysis of samples: follow either method below. 1) Tissue Samples Homogenize tissue samples in 1.5 mL of RNA-Solv ® Reagent per 100
RNASafer® Protocol for Total RNA Isolation
buffer. Homogenize the tissue with a rotor-stator homogenizer. For RNA isolation using Midi and Maxiprep kit, follow the standard protocol in the user manual. 4) Follow standard protocol for E.Z.N.A.® Total RNA Kits in user manuals.
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
25 mM Tris pH=8.0 1M 1,25 ml MPS2 for 10 ml 0,2 N NaOH 10N 200 ml 1% SDS 10% 1 ml H2O 8,8 ml MPS3 for 100 ml 5 M potassium acetate 60 ml glacial acetic acid 11,5 ml H2O 28,5ml
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