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文献和实验Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
of binding". For this reason it is essential to include an internal control antibody for chromosome morphology and antibody access. For this internal control I use a mouse monoclonal anti-Modulo antibody (Perrin et al ., 1998; the antibody was obtained
," made up of fragments of up to seven nucleosomes in length, is incubated with an antiserum directed against the histone modification of interest. The antibody-bound fraction is separated from the unbound fraction and, after extraction of genomic DNA from the bound
," made up of fragments of up to seven nucleosomes in length, is incubated with an antiserum directed against the histone modification of interest. The antibody-bound fraction is separated from the unbound fraction and, after extraction of genomic DNA from the bound
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